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PDBsum entry 5c21
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Protein transport
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PDB id
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5c21
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References listed in PDB file
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Key reference
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Title
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Crystal structure of a soluble fragment of the membrane fusion protein hlyd in a type i secretion system of gram-Negative bacteria.
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Authors
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J.S.Kim,
S.Song,
M.Lee,
S.Lee,
K.Lee,
N.C.Ha.
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Ref.
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Structure, 2016,
24,
477-485.
[DOI no: ]
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PubMed id
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Abstract
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The protein toxin HlyA of Escherichia coli is exported without a periplasmic
intermediate by the type I secretion system (T1SS). The T1SS is composed of an
inner membrane ABC transporter HlyB, an outer-membrane channel protein TolC, and
a membrane fusion protein HlyD. However, the assembly of the T1SS remains to be
elucidated. In this study, we determine the crystal structure of a part of the
C-terminal periplasmic domain of HlyD. The long α-helical domain consisting of
three α helices and a lipoyl domain was identified in the crystal structure.
Based on the HlyD structure, we modeled the hexameric assembly of HlyD with a
long α-helical barrel, which formed a complex with TolC in an intermeshing
cogwheel-to-cogwheel manner, as observed in tripartite RND-type drug efflux
pumps. These observations provide a structural blueprint for understanding the
type I secretion system in pathogenic Gram-negative bacteria.
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