PDBsum entry 5buz

Transport protein

PDB id: 5buz

Contents

Protein chains

605 a.a.

324 a.a.

38 a.a.

40 a.a.

PDB id:

5buz

Name:

Transport protein

Title:

Crystal structure of a complex between the snare vam3 and the hops vps33-vps16 subcomplex from chaetomium thermophilum

Structure:

Sm (sec1/munc18-like) protein. Chain: a, d. Synonym: vps33. Engineered: yes. Putative vacuolar protein sorting-associated protein. Chain: b, e. Synonym: vps16. Engineered: yes. Snap receptor-like protein.

Source:

Chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719). Organism_taxid: 759272. Strain: dsm 1495 / cbs 144.50 / imi 039719. Gene: ctht_0057760. Expressed in: escherichia coli. Expression_system_taxid: 469008. Chaetomium thermophilum. Gene: ctht_0026760.

Resolution:

3.10Å R-factor: 0.196 R-free: 0.233

Authors:

R.W.Baker,P.D.Jeffrey,F.M.Hughson

Key ref:

R.W.Baker et al. (2015). A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly. Science, 349, 1111-1114. PubMed id: 26339030 DOI: 10.1126/science.aac7906

Date:

04-Jun-15 Release date: 05-Aug-15

Protein chains

G0SCM5  (G0SCM5_CHATD) -  Small conjugating protein ligase-like protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 806 a.a.

Struc: 605 a.a.

Protein chains

G0S6M7  (G0S6M7_CHATD) -  Probable vacuolar protein sorting-associated protein 16 homolog from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 816 a.a.

Struc: 324 a.a.*

Protein chain

G0S236  (G0S236_CHATD) -  SNAP receptor-like protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 273 a.a.

Struc: 38 a.a.

Protein chain

G0S236  (G0S236_CHATD) -  SNAP receptor-like protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719)

Seq: 273 a.a.

Struc: 40 a.a.

* PDB and UniProt seqs differ at 19 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F: E.C.?

DOI no: 10.1126/science.aac7906

Science 349:1111-1114 (2015)

PubMed id: 26339030

A direct role for the Sec1/Munc18-family protein Vps33 as a template for SNARE assembly.

R.W.Baker, P.D.Jeffrey, M.Zick, B.P.Phillips, W.T.Wickner, F.M.Hughson.

ABSTRACT

Fusion of intracellular transport vesicles requires soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) and Sec1/Munc18-family (SM) proteins. Membrane-bridging SNARE complexes are critical for fusion, but their spontaneous assembly is inefficient and may require SM proteins in vivo. We report x-ray structures of Vps33, the SM subunit of the yeast homotypic fusion and vacuole protein-sorting (HOPS) complex, bound to two individual SNAREs. The two SNAREs, one from each membrane, are held in the correct orientation and register for subsequent complex assembly. Vps33 and potentially other SM proteins could thus act as templates for generating partially zipped SNARE assembly intermediates. HOPS was essential to mediate SNARE complex assembly at physiological SNARE concentrations. Thus, Vps33 appears to catalyze SNARE complex assembly through specific SNARE motif recognition.