PDBsum entry 5b32

DNA binding protein

PDB id: 5b32

Contents

Protein chains

97 a.a.

78 a.a.

108 a.a.

93 a.a.

83 a.a.

104 a.a.

DNA/RNA

Metals

_CL ×4

_MN ×10

Waters ×88

PDB id:

5b32

Name:

DNA binding protein

Title:

The crystal structure of the heterotypic h2az/h2a nucleosome with h3.3.

Structure:

Histone h3.3. Chain: a, e. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes. Histone h2a type 1-b/e. Chain: c. Synonym: histone h2a.2,histone h2a/a,histone h2a/m.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: h3f3a, h3.3a, h3f3, pp781, h3f3b, h3.3b. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: hist1h4a, h4/a, h4fa, hist1h4b, h4/i, h4fi, hist1h4c, h4/g, h4fg, hist1h4d, h4/b, h4fb, hist1h4e, h4/j, h4fj, hist1h4f, h4/c, h4fc, hist1h4h, h4/h, h4fh, hist1h4i, h4/m, h4fm, hist1h4j, h4/e,

Resolution:

2.35Å R-factor: 0.228 R-free: 0.259

Authors:

N.Horikoshi,H.Taguchi,Y.Arimura,H.Kurumizaka

Key ref:

N.Horikoshi et al. (2016). Crystal structures of heterotypic nucleosomes contain histones h2a.Z and h2a.. Open biology, 6, . PubMed id: 27358293

Date:

08-Feb-16 Release date: 03-Aug-16

Protein chains

P84243  (H33_HUMAN) -  Histone H3.3 from Homo sapiens

Seq: 136 a.a.

Struc: 97 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 78 a.a.

Protein chain

P04908  (H2A1B_HUMAN) -  Histone H2A type 1-B/E from Homo sapiens

Seq: 130 a.a.

Struc: 108 a.a.

Protein chains

P06899  (H2B1J_HUMAN) -  Histone H2B type 1-J from Homo sapiens

Seq: 126 a.a.

Struc: 93 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 83 a.a.

Protein chain

P0C0S5  (H2AZ_HUMAN) -  Histone H2A.Z from Homo sapiens

Seq: 128 a.a.

Struc: 104 a.a.

DNA/RNA chains

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 146 bases

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 146 bases