PDBsum entry 5b31

DNA binding protein

PDB id: 5b31

Contents

Protein chains

97 a.a.

78 a.a.

107 a.a.

93 a.a.

83 a.a.

104 a.a.

DNA/RNA

Metals

_CL ×4

_MN ×8

Waters ×292

PDB id:

5b31

Name:

DNA binding protein

Title:

The crystal structure of the heterotypic h2az/h2a nucleosome with h3.1.

Structure:

Histone h3.1. Chain: a, e. Synonym: histone h3/a,histone h3/b,histone h3/c,histone h3/d,histone h3/f,histone h3/h,histone h3/i,histone h3/j,histone h3/k,histone h3/l. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hist1h3a, h3fa, hist1h3b, h3fl, hist1h3c, h3fc, hist1h3d, h3fb, hist1h3e, h3fd, hist1h3f, h3fi, hist1h3g, h3fh, hist1h3h, h3fk, hist1h3i, h3ff, hist1h3j, h3fj. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Gene: hist1h4a, h4/a, h4fa, hist1h4b, h4/i, h4fi, hist1h4c, h4/g,

Resolution:

2.20Å R-factor: 0.228 R-free: 0.271

Authors:

N.Horikoshi,H.Taguchi,Y.Arimura,H.Kurumizaka

Key ref:

N.Horikoshi et al. (2016). Crystal structures of heterotypic nucleosomes contain histones h2a.Z and h2a.. Open biology, 6, . PubMed id: 27358293

Date:

08-Feb-16 Release date: 03-Aug-16

Protein chains

P68431  (H31_HUMAN) -  Histone H3.1 from Homo sapiens

Seq: 136 a.a.

Struc: 97 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 78 a.a.

Protein chain

P04908  (H2A1B_HUMAN) -  Histone H2A type 1-B/E from Homo sapiens

Seq: 130 a.a.

Struc: 107 a.a.

Protein chains

P06899  (H2B1J_HUMAN) -  Histone H2B type 1-J from Homo sapiens

Seq: 126 a.a.

Struc: 93 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 83 a.a.

Protein chain

P0C0S5  (H2AZ_HUMAN) -  Histone H2A.Z from Homo sapiens

Seq: 128 a.a.

Struc: 104 a.a.

DNA/RNA chains

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 146 bases

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 146 bases