PDBsum entry 5ayq

Metal transport

PDB id: 5ayq

Contents

Protein chains

118 a.a.

Waters ×230

PDB id:

5ayq

Name:

Metal transport

Title:

Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of vgsc beta subunits

Structure:

Sodium channel subunit beta-4. Chain: a, b. Fragment: unp residues 30-160. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: scn4b, gm1471. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.70Å R-factor: 0.205 R-free: 0.231

Authors:

H.Shimizu

Key ref:

H.Shimizu et al. (2016). Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of voltage-gated sodium channel β subunits. Sci Rep, 6, 26618. PubMed id: 27216889 DOI: 10.1038/srep26618

Date:

01-Sep-15 Release date: 08-Jun-16

Protein chains

Q7M729  (SCN4B_MOUSE) -  Sodium channel subunit beta-4 from Mus musculus

Seq: 228 a.a.

Struc: 118 a.a.

DOI no: 10.1038/srep26618

Sci Rep 6:26618 (2016)

PubMed id: 27216889

Structure-based site-directed photo-crosslinking analyses of multimeric cell-adhesive interactions of voltage-gated sodium channel β subunits.

H.Shimizu, H.Miyazaki, N.Ohsawa, S.Shoji, Y.Ishizuka-Katsura, A.Tosaki, F.Oyama, T.Terada, K.Sakamoto, M.Shirouzu, S.Sekine, N.Nukina, S.Yokoyama.

ABSTRACT

The β1, β2, and β4 subunits of voltage-gated sodium channels reportedly function as cell adhesion molecules. The present crystallographic analysis of the β4 extracellular domain revealed an antiparallel arrangement of the β4 molecules in the crystal lattice. The interface between the two antiparallel β4 molecules is asymmetric, and results in a multimeric assembly. Structure-based mutagenesis and site-directed photo-crosslinking analyses of the β4-mediated cell-cell adhesion revealed that the interface between the antiparallel β4 molecules corresponds to that in the trans homophilic interaction for the multimeric assembly of β4 in cell-cell adhesion. This trans interaction mode is also employed in the β1-mediated cell-cell adhesion. Moreover, the β1 gene mutations associated with generalized epilepsy with febrile seizures plus (GEFS+) impaired the β1-mediated cell-cell adhesion, which should underlie the GEFS+ pathogenesis. Thus, the structural basis for the β-subunit-mediated cell-cell adhesion has been established.