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PDBsum entry 5avd
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PDB id:
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Isomerase
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Title:
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The 0.86 angstrom structure of elastase crystallized in high-strength agarose hydrogel
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Structure:
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Chymotrypsin-like elastase family member 1. Chain: a. Synonym: elastase-1. Engineered: yes
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Source:
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Sus scrofa. Pig. Organism_taxid: 9823. Gene: cela1, ela1. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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0.86Å
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R-factor:
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0.129
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R-free:
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0.140
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Authors:
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S.Sugiyama,N.Shimizu,M.Maruyama,G.Sazaki,H.Adachi,K.Takano, S.Murakami,T.Inoue,Y.Mori,H.Matsumura
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Key ref:
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S.Sugiyama
et al.
(2012).
Growth of protein crystals in hydrogels prevents osmotic shock.
J Am Chem Soc,
134,
5786-5789.
PubMed id:
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Date:
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15-Jun-15
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Release date:
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08-Jul-15
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PROCHECK
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Headers
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References
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P00772
(CELA1_PIG) -
Chymotrypsin-like elastase family member 1 from Sus scrofa
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Seq:
Struc:
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266 a.a.
240 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Enzyme class:
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E.C.3.4.21.36
- pancreatic elastase.
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Reaction:
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Hydrolysis of proteins, including elastin. Preferential cleavage: Ala-|-Xaa.
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J Am Chem Soc
134:5786-5789
(2012)
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PubMed id:
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Growth of protein crystals in hydrogels prevents osmotic shock.
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S.Sugiyama,
M.Maruyama,
G.Sazaki,
M.Hirose,
H.Adachi,
K.Takano,
S.Murakami,
T.Inoue,
Y.Mori,
H.Matsumura.
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ABSTRACT
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High-throughput protein X-ray crystallography offers a significant opportunity
to facilitate drug discovery. The most reliable approach is to determine the
three-dimensional structure of the protein-ligand complex by soaking the ligand
in apo crystals. However, protein apo crystals produced by conventional
crystallization in a solution are fatally damaged by osmotic shock during
soaking. To overcome this difficulty, we present a novel technique for growing
protein crystals in a high-concentration hydrogel that is completely gellified
and exhibits high strength. This technique allowed us essentially to increase
the mechanical stability of the crystals, preventing serious damage to the
crystals caused by osmotic shock. Thus, this method may accelerate
structure-based drug discoveries.
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