UniProt functional annotation for Q8C9A2



	UniProt functional annotation for Q8C9A2

UniProt code: Q8C9A2.

Organism: Mus musculus (Mouse).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.

Function: Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine. Active against both single-stranded and double-stranded RNAs. Has strong preference for single-stranded RNAs (ssRNAs) toward double- stranded RNAs (dsRNAs). Cleaves mRNAs and tRNAs containing inosine. Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3'. Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing. Binds branched DNA structures. {ECO:0000250|UniProtKB:Q8N8Q3}.

Cofactor: Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:12853604};

Subunit: Monomer. Interacts with PABPC1; the interaction is RNA- dependent and stimulates ENDOV activity. {ECO:0000250|UniProtKB:Q8N8Q3}.

Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:Q8N8Q3}. Nucleus, nucleolus {ECO:0000250|UniProtKB:Q8N8Q3}. Cytoplasm, Stress granule {ECO:0000250|UniProtKB:Q8N8Q3}. Note=Relocalizes to cytoplasmic stress granules upon cellular stress where it colocalizes with PABPC1. {ECO:0000250|UniProtKB:Q8N8Q3}.

Tissue specificity: Highest levels detected in liver with high levels also found in heart, kidney and testis. Expressed at low levels in brain. {ECO:0000269|PubMed:12853604}.

Miscellaneous: Was initially characterized as an endodeoxyribonuclease involved in DNA repair (PubMed:12853604). While it shows some weak endodeoxyribonuclease activity in vitro, such activity probably does not exist in vivo. {ECO:0000305|PubMed:12853604}.

Similarity: Belongs to the endonuclease V family. {ECO:0000305}.

Sequence caution: Sequence=BAC39216.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Mus musculus (Mouse).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.



Function:		Endoribonuclease that specifically cleaves inosine-containing RNAs: cleaves RNA at the second phosphodiester bond 3' to inosine. Active against both single-stranded and double-stranded RNAs. Has strong preference for single-stranded RNAs (ssRNAs) toward double- stranded RNAs (dsRNAs). Cleaves mRNAs and tRNAs containing inosine. Also able to cleave structure-specific dsRNA substrates containing the specific sites 5'-IIUI-3' and 5'-UIUU-3'. Inosine is present in a number of RNAs following editing; the function of inosine-specific endoribonuclease is still unclear: it could either play a regulatory role in edited RNAs, or be involved in antiviral response by removing the hyperedited long viral dsRNA genome that has undergone A-to-I editing. Binds branched DNA structures. {ECO:0000250\|UniProtKB:Q8N8Q3}.


Cofactor:		Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269\|PubMed:12853604};
Subunit:		Monomer. Interacts with PABPC1; the interaction is RNA- dependent and stimulates ENDOV activity. {ECO:0000250\|UniProtKB:Q8N8Q3}.
Subcellular location:		Cytoplasm {ECO:0000250\|UniProtKB:Q8N8Q3}. Nucleus, nucleolus {ECO:0000250\|UniProtKB:Q8N8Q3}. Cytoplasm, Stress granule {ECO:0000250\|UniProtKB:Q8N8Q3}. Note=Relocalizes to cytoplasmic stress granules upon cellular stress where it colocalizes with PABPC1. {ECO:0000250\|UniProtKB:Q8N8Q3}.
Tissue specificity:		Highest levels detected in liver with high levels also found in heart, kidney and testis. Expressed at low levels in brain. {ECO:0000269\|PubMed:12853604}.
Miscellaneous:		Was initially characterized as an endodeoxyribonuclease involved in DNA repair (PubMed:12853604). While it shows some weak endodeoxyribonuclease activity in vitro, such activity probably does not exist in vivo. {ECO:0000305\|PubMed:12853604}.
Similarity:		Belongs to the endonuclease V family. {ECO:0000305}.
Sequence caution:		Sequence=BAC39216.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};