PDBsum entry 5ams

Chaperone

PDB id: 5ams

Contents

Protein chains

392 a.a.

Waters ×1

PDB id:

5ams

Name:

Chaperone

Title:

Crystal structure of sqt1

Structure:

Ribosome assembly protein sqt1. Chain: a, b. Synonym: sqt1. Engineered: yes

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

3.35Å R-factor: 0.174 R-free: 0.222

Authors:

F.Frenois,P.Legrand,S.Fribourg

Key ref:

F.Frénois et al. (2016). Sqt1p is an eight-bladed WD40 protein. Acta Crystallogr F Struct Biol Commun, 72, 59-64. PubMed id: 26750486 DOI: 10.1107/S2053230X15024097

Date:

31-Aug-15 Release date: 13-Jan-16

Protein chains

P35184  (SQT1_YEAST) -  Ribosome assembly protein SQT1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 431 a.a.

Struc: 392 a.a.

DOI no: 10.1107/S2053230X15024097

Acta Crystallogr F Struct Biol Commun 72:59-64 (2016)

PubMed id: 26750486

Sqt1p is an eight-bladed WD40 protein.

F.Frénois, P.Legrand, S.Fribourg.

ABSTRACT

Ribosome biogenesis in eukaryotes is a complex and highly orchestrated process involving more than 200 accessory factors in addition to ribosomal RNAs and ribosomal proteins. Among the many factors involved, Sqt1p has been reported to specifically bind to uL16 and to act as a chaperone. The crystal structure of full-length Sqt1p from the yeast Saccharomyces cerevisiae has been solved at 3.35 Å resolution. A SAD experiment at the Se K edge and an S-SAD experiment on the same selenomethionine-substituted protein crystal allowed unambiguous positioning of the selenomethionine and Cys residues. On the basis of the atomic structure of Sqt1p, the potential residues involved in uL16 interaction were identified and tested.