PDBsum entry 5amf

Histone-binding protein

PDB id: 5amf

Contents

Protein chains

121 a.a.

Ligands

TWL

Metals

_NA

Waters ×239

PDB id:

5amf

Name:

Histone-binding protein

Title:

Crystal structure of the bromodomain of human surface epitope engineered brd1a in complex with 3d consortium fragment ethyl 4,5,6, 7- tetrahydro-1h-indazole-5-carboxylate (sgc - diamond i04-1 fragment screening)

Structure:

Bromodomain-containing protein 1. Chain: a, b. Fragment: bromodomain and phd finger, residues 556-688. Synonym: br140-like protein, bromodomain and phd finger-containing protein 2, brd1a, br140-like protein. Engineered: yes. Mutation: yes. Other_details: surface epitope engineered p566e, v569r

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: r3-prare2.

Resolution:

1.75Å R-factor: 0.177 R-free: 0.213

Authors:

N.M.Pearce,M.Fairhead,C.Strain-Damerell,R.Talon,N.Wright,J.T.Ng, A.Bradley,O.Cox,D.Bowkett,P.Collins,J.Brandao-Neto,A.Douangamath, T.Krojer,N.Burgess-Brown,P.Brennan,C.H.Arrowsmith,E.Edwards, C.Bountra,F.Von Delft

Key ref:

N.M.Pearce et al. Crystal structure of the bromodomain of human surface epitope engineered brd1a in complex with 3d consortiu fragment ethyl 4,5,6,7-Tetrahydro-1h- Indazole-5-Carboxylate. To be published, .

Date:

10-Mar-15 Release date: 18-Mar-15

Protein chains

O95696  (BRD1_HUMAN) -  Bromodomain-containing protein 1 from Homo sapiens

Seq: 1058 a.a.

Struc: 121 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)