 |
PDBsum entry 5afu
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Motor protein
|
PDB id
|
|
|
|
5afu
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
361 a.a.
|
|
|
|
|
|
|
|
|
|
|
350 a.a.
|
|
|
|
|
|
|
|
|
|
|
275 a.a.
|
|
|
|
|
|
|
|
|
|
|
(+ 2 more)
370 a.a.
|
|
|
|
|
|
|
|
|
|
|
370 a.a.
|
|
|
|
|
|
|
|
|
|
|
369 a.a.
|
|
|
|
|
|
|
|
|
|
|
275 a.a.
|
|
|
|
|
|
|
|
|
|
|
270 a.a.
|
|
|
|
|
|
|
|
|
|
|
587 a.a.
|
|
|
|
|
|
|
|
|
|
|
65 a.a.
|
|
|
|
|
|
|
|
|
|
|
87 a.a.
|
|
|
|
|
|
|
|
|
|
|
168 a.a.
|
|
|
|
|
|
|
|
|
|
|
165 a.a.
|
|
|
|
|
|
|
|
|
|
|
243 a.a.
|
|
|
|
|
|
|
|
|
|
|
52 a.a.
|
|
|
|
|
|
|
|
|
|
|
48 a.a.
|
|
|
|
|
|
|
|
|
|
|
71 a.a.
|
|
|
|
|
|
|
|
|
|
|
31 a.a.
|
|
|
|
|
|
|
|
|
|
|
20 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
The structure of the dynactin complex and its interaction with dynein.
|
|
|
Authors
|
|
L.Urnavicius,
K.Zhang,
A.G.Diamant,
C.Motz,
M.A.Schlager,
M.Yu,
N.A.Patel,
C.V.Robinson,
A.P.Carter.
|
|
|
Ref.
|
|
Science, 2015,
347,
1441-1446.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Dynactin is an essential cofactor for the microtubule motor cytoplasmic
dynein-1. We report the structure of the 23-subunit dynactin complex by
cryo-electron microscopy to 4.0 angstroms. Our reconstruction reveals how
dynactin is built around a filament containing eight copies of the actin-related
protein Arp1 and one of β-actin. The filament is capped at each end by distinct
protein complexes, and its length is defined by elongated peptides that emerge
from the α-helical shoulder domain. A further 8.2 angstrom structure of the
complex between dynein, dynactin, and the motility-inducing cargo adaptor
Bicaudal-D2 shows how the translational symmetry of the dynein tail matches that
of the dynactin filament. The Bicaudal-D2 coiled coil runs between dynein and
dynactin to stabilize the mutually dependent interactions between all three
components.
|
|
|
|
|
|
|
