PDBsum entry 5afu

Motor protein

PDB id: 5afu

Contents

Protein chains

361 a.a.

350 a.a.

275 a.a.

(+ 2 more) 370 a.a.

370 a.a.

369 a.a.

275 a.a.

270 a.a.

587 a.a.

65 a.a.

87 a.a.

168 a.a.

165 a.a.

243 a.a.

52 a.a.

48 a.a.

71 a.a.

31 a.a.

20 a.a.

Ligands

ADP ×9

ATP

PDB id:

5afu

Name:

Motor protein

Title:

Cryo-em structure of dynein tail-dynactin-bicd2n complex

Structure:

Dynein tail. Chain: 1. Dynein tail. Chain: 2. Dynein tail. Chain: 3, 4. Dynein tail. Chain: 5, 6. Dynactin.

Source:

Sus scrofa. Pig. Organism_taxid: 9823. Organ: brain. Organ: brain

Authors:

L.Urnavicius,K.Zhang,A.G.Diamant,C.Motz,M.A.Schlager,M.Yu,N.A.Patel, C.V.Robinson,A.P.Carter

Key ref:

L.Urnavicius et al. (2015). The structure of the dynactin complex and its interaction with dynein. Science, 347, 1441-1446. PubMed id: 25814576 DOI: 10.1126/science.aaa4080

Date:

26-Jan-15 Release date: 11-Mar-15

Protein chains

No UniProt id for this chain

Struc: 361 a.a.

Protein chains

A0A0J9X2A1  (A0A0J9X2A1_PIG) -  Dynein tail from Sus scrofa

Seq: 350 a.a.

Struc: 350 a.a.

Protein chains

No UniProt id for this chain

Struc: 275 a.a.

Protein chains

F2Z5G5  (F2Z5G5_PIG) -  Alpha-centractin from Sus scrofa

Seq: 349 a.a.

Struc: 370 a.a.*

Protein chain

Q6QAQ1  (ACTB_PIG) -  Actin, cytoplasmic 1 from Sus scrofa

Seq: 375 a.a.

Struc: 370 a.a.

Protein chain

I3LHK5  (I3LHK5_PIG) -  Actin-related protein 10 from Sus scrofa

Seq: 417 a.a.

Struc: 369 a.a.

Protein chain

A0PFK5  (A0PFK5_PIG) -  F-actin-capping protein subunit alpha-1 from Sus scrofa

Seq: 286 a.a.

Struc: 275 a.a.

Protein chain

D2JYW4  (D2JYW4_PIG) -  F-actin-capping protein subunit beta from Sus scrofa

Seq: 277 a.a.

Struc: 270 a.a.

Protein chains

No UniProt id for this chain

Struc: 587 a.a.

Protein chains

No UniProt id for this chain

Struc: 65 a.a.

Protein chains

No UniProt id for this chain

Struc: 87 a.a.

Protein chain

D0G6S1  (D0G6S1_PIG) -  Dynactin subunit 6 from Sus scrofa

Seq: 190 a.a.

Struc: 168 a.a.*

Protein chain

A0A286ZK88  (A0A286ZK88_PIG) -  Dynactin subunit 5 from Sus scrofa

Seq: 182 a.a.

Struc: 165 a.a.*

Protein chain

No UniProt id for this chain

Struc: 243 a.a.

Protein chains

No UniProt id for this chain

Struc: 52 a.a.

Protein chain

A0A0J9X292  (A0A0J9X292_PIG) -  Dynactin from Sus scrofa

Seq: 48 a.a.

Struc: 48 a.a.

Protein chain

A0A0J9X293  (A0A0J9X293_PIG) -  Dynactin subunit 2 from Sus scrofa

Seq: 71 a.a.

Struc: 71 a.a.

Protein chain

A0A0J9X299  (A0A0J9X299_PIG) -  Dynactin from Sus scrofa

Seq: 31 a.a.

Struc: 31 a.a.

Protein chain

A0A0J9X295  (A0A0J9X295_PIG) -  Dynactin from Sus scrofa

Seq: 20 a.a.

Struc: 20 a.a.

* PDB and UniProt seqs differ at 41 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain H: E.C.3.6.4.- - ?????

DOI no: 10.1126/science.aaa4080

Science 347:1441-1446 (2015)

PubMed id: 25814576

The structure of the dynactin complex and its interaction with dynein.

L.Urnavicius, K.Zhang, A.G.Diamant, C.Motz, M.A.Schlager, M.Yu, N.A.Patel, C.V.Robinson, A.P.Carter.

ABSTRACT

Dynactin is an essential cofactor for the microtubule motor cytoplasmic dynein-1. We report the structure of the 23-subunit dynactin complex by cryo-electron microscopy to 4.0 angstroms. Our reconstruction reveals how dynactin is built around a filament containing eight copies of the actin-related protein Arp1 and one of β-actin. The filament is capped at each end by distinct protein complexes, and its length is defined by elongated peptides that emerge from the α-helical shoulder domain. A further 8.2 angstrom structure of the complex between dynein, dynactin, and the motility-inducing cargo adaptor Bicaudal-D2 shows how the translational symmetry of the dynein tail matches that of the dynactin filament. The Bicaudal-D2 coiled coil runs between dynein and dynactin to stabilize the mutually dependent interactions between all three components.