UniProt functional annotation for P62168



	UniProt functional annotation for P62168

UniProt code: P62168.

Organism: Rattus norvegicus (Rat).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.

Function: Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel. {ECO:0000269|PubMed:17502602}.

Subunit: Monomer (PubMed:25979333). Interacts with KCND2 (By similarity). Interacts in a calcium-independent manner with PI4KB, but only if myristoylated (PubMed:11526106). This binding competes with CALN2/CABP7 binding to PI4KB (PubMed:11526106). Interacts in a calcium- dependent manner with PICK1 (via AH domain) (PubMed:19109914). Interacts with ARF1, ARF3, ARF5 and ARF6 (By similarity). Interacts with IL1RAPL1 (By similarity). {ECO:0000250|UniProtKB:P62166, ECO:0000269|PubMed:11526106, ECO:0000269|PubMed:19109914, ECO:0000269|PubMed:25979333}.

Subcellular location: Golgi apparatus {ECO:0000269|PubMed:11526106}. Cell junction, synapse, postsynaptic density {ECO:0000305}. Cytoplasm, perinuclear region {ECO:0000250|UniProtKB:P62166}. Cytoplasm {ECO:0000269|PubMed:11526106}. Cell membrane {ECO:0000269|PubMed:11526106}. Membrane {ECO:0000250|UniProtKB:P62166, ECO:0000269|PubMed:10514519}; Lipid-anchor {ECO:0000250|UniProtKB:P62166}. Note=Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions. {ECO:0000250|UniProtKB:P62166, ECO:0000269|PubMed:11526106, ECO:0000305}.

Domain: Binds 3 calcium ions via the second, third and fourth EF-hand. EF-2 and EF-3 bind both magnesium and calcium, while EF-4 binds only calcium. At the resting state, magnesium is bound to EF-2 and EF-3 and upon activation, both sites bind calcium simultaneously while EF-4 is the last one to be occupied.

Miscellaneous: Fourfold reduction in calcium affinity for NCS1/FREQ in the presence of magnesium.

Similarity: Belongs to the recoverin family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Rattus norvegicus (Rat).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus.



Function:		Neuronal calcium sensor, regulator of G protein-coupled receptor phosphorylation in a calcium dependent manner. Directly regulates GRK1 (RHOK), but not GRK2 to GRK5. Can substitute for calmodulin. Stimulates PI4KB kinase activity. Involved in long-term synaptic plasticity through its interaction with PICK1. May also play a role in neuron differentiation through inhibition of the activity of N- type voltage-gated calcium channel. {ECO:0000269\|PubMed:17502602}.


Subunit:		Monomer (PubMed:25979333). Interacts with KCND2 (By similarity). Interacts in a calcium-independent manner with PI4KB, but only if myristoylated (PubMed:11526106). This binding competes with CALN2/CABP7 binding to PI4KB (PubMed:11526106). Interacts in a calcium- dependent manner with PICK1 (via AH domain) (PubMed:19109914). Interacts with ARF1, ARF3, ARF5 and ARF6 (By similarity). Interacts with IL1RAPL1 (By similarity). {ECO:0000250\|UniProtKB:P62166, ECO:0000269\|PubMed:11526106, ECO:0000269\|PubMed:19109914, ECO:0000269\|PubMed:25979333}.
Subcellular location:		Golgi apparatus {ECO:0000269\|PubMed:11526106}. Cell junction, synapse, postsynaptic density {ECO:0000305}. Cytoplasm, perinuclear region {ECO:0000250\|UniProtKB:P62166}. Cytoplasm {ECO:0000269\|PubMed:11526106}. Cell membrane {ECO:0000269\|PubMed:11526106}. Membrane {ECO:0000250\|UniProtKB:P62166, ECO:0000269\|PubMed:10514519}; Lipid-anchor {ECO:0000250\|UniProtKB:P62166}. Note=Associated with Golgi stacks. Post-synaptic densities of dendrites, and in the pre-synaptic nerve terminal at neuromuscular junctions. {ECO:0000250\|UniProtKB:P62166, ECO:0000269\|PubMed:11526106, ECO:0000305}.
Domain:		Binds 3 calcium ions via the second, third and fourth EF-hand. EF-2 and EF-3 bind both magnesium and calcium, while EF-4 binds only calcium. At the resting state, magnesium is bound to EF-2 and EF-3 and upon activation, both sites bind calcium simultaneously while EF-4 is the last one to be occupied.
Miscellaneous:		Fourfold reduction in calcium affinity for NCS1/FREQ in the presence of magnesium.
Similarity:		Belongs to the recoverin family. {ECO:0000305}.