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PDBsum entry 5afp
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protein metals Protein-protein interface(s) links
Signaling protein PDB id
5afp

 

 

 

 

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Contents
Protein chains
173 a.a.
12 a.a.
Metals
_NA ×2
_CA ×6
Waters ×26
PDB id:
5afp
Name: Signaling protein
Title: Neuronal calcium sensor-1 (ncs-1)from rattus norvegicus complex with rhodopsin kinase peptide from homo sapiens
Structure: Neuronal calcium sensor 1. Chain: a, b. Synonym: ncs-1, frequenin homolog, frequenin-like protein, frequenin- like ubiquitous protein. Engineered: yes. Rhodopsin kinase. Chain: c, d. Fragment: residues 1-25. Synonym: rk, g protein-coupled receptor kinase 1.
Source: Rattus norvegicus. Norway rat. Organism_taxid: 10116. Cell: neuron. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Homo sapiens. Human.
Resolution:
2.30Å     R-factor:   0.227     R-free:   0.255
Authors: M.Saleem,V.Karuppiah,S.Pandalaneni,R.Burgoyne,J.P.Derrick,L.Y.Lian
Key ref: S.Pandalaneni et al. (2015). Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions. J Biol Chem, 290, 18744-18756. PubMed id: 25979333 DOI: 10.1074/jbc.M114.627059
Date:
23-Jan-15     Release date:   18-Mar-15    
Supersedes: 4uyc
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P62168  (NCS1_RAT) -  Neuronal calcium sensor 1 from Rattus norvegicus
Seq:
Struc: 		190 a.a.
173 a.a.
Protein chains
Pfam   ArchSchema ?
Q15835  (RK_HUMAN) -  Rhodopsin kinase GRK1 from Homo sapiens
Seq:
Struc: 		 
Seq:
Struc: 		563 a.a.
12 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chains C, D: E.C.2.7.11.14  - rhodopsin kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction:
1. L-seryl-[rhodopsin] + ATP = O-phospho-L-seryl-[rhodopsin] + ADP + H+
2. L-threonyl-[rhodopsin] + ATP = O-phospho-L-threonyl-[rhodopsin] + ADP + H+
L-seryl-[rhodopsin]
 + ATP
 = O-phospho-L-seryl-[rhodopsin]
 + ADP
 + H(+)
L-threonyl-[rhodopsin]
 + ATP
 = O-phospho-L-threonyl-[rhodopsin]
 + ADP
 + H(+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
DOI no: 10.1074/jbc.M114.627059 J Biol Chem 290:18744-18756 (2015)
PubMed id: 25979333  
 
 
Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1) Peptides Using Different Modes of Interactions.
S.Pandalaneni, V.Karuppiah, M.Saleem, L.P.Haynes, R.D.Burgoyne, O.Mayans, J.P.Derrick, L.Y.Lian.
 
  ABSTRACT  
 
No abstract given.

 

 

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