PDBsum entry 5af6

Signaling protein

PDB id: 5af6

Contents

Protein chains

76 a.a.

28 a.a.

29 a.a.

28 a.a.

27 a.a.

Metals

_ZN ×5

PDB id:

5af6

Name:

Signaling protein

Title:

Structure of lys33-linked diub bound to trabid nzf1

Structure:

Ubiquitin. Chain: a, b, c, d, e. Engineered: yes. Mutation: yes. Other_details: isopeptide bonds across between chains, a76 c and d33 nz, b76 c and a33 nz, c76c and b33 nz, e76 c and c33 nz. Isopeptide bond across the asu for, d76 c and e33 nz symm. Trabid. Chain: f, g, h, i, j.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008. Expression_system_variant: rosettaii.

Resolution:

3.40Å R-factor: 0.182 R-free: 0.222

Authors:

M.A.Michel,P.R.Elliott,K.N.Swatek,M.Simicek,J.N.Pruneda,J.L.Wagstaff, S.M.V.Freund,D.Komander

Key ref:

M.A.Michel et al. (2015). Assembly and specific recognition of k29- and k33-linked polyubiquitin. Mol Cell, 58, 95. PubMed id: 25752577 DOI: 10.1016/j.molcel.2015.01.042

Date:

19-Jan-15 Release date: 25-Mar-15

Protein chains

P0CG48  (UBC_HUMAN) -  Polyubiquitin-C from Homo sapiens

Seq: 685 a.a.

Struc: 76 a.a.*

Protein chains

Q9UGI0  (ZRAN1_HUMAN) -  Ubiquitin thioesterase ZRANB1 from Homo sapiens

Seq: 708 a.a.

Struc: 28 a.a.

Protein chain

Q9UGI0  (ZRAN1_HUMAN) -  Ubiquitin thioesterase ZRANB1 from Homo sapiens

Seq: 708 a.a.

Struc: 29 a.a.

Protein chain

Q9UGI0  (ZRAN1_HUMAN) -  Ubiquitin thioesterase ZRANB1 from Homo sapiens

Seq: 708 a.a.

Struc: 28 a.a.

Protein chain

Q9UGI0  (ZRAN1_HUMAN) -  Ubiquitin thioesterase ZRANB1 from Homo sapiens

Seq: 708 a.a.

Struc: 27 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class 2: Chains A, B, C, D, E: E.C.?
• Enzyme class 3: Chains F, G, H, I, J: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

DOI no: 10.1016/j.molcel.2015.01.042

Mol Cell 58:95 (2015)

PubMed id: 25752577

Assembly and specific recognition of k29- and k33-linked polyubiquitin.

M.A.Michel, P.R.Elliott, K.N.Swatek, M.Simicek, J.N.Pruneda, J.L.Wagstaff, S.M.Freund, D.Komander.

ABSTRACT

No abstract given.