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PDBsum entry 5af4

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protein ligands Protein-protein interface(s) links
Signaling protein PDB id
5af4

 

 

 

 

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JSmol PyMol  
Contents
Protein chains
(+ 2 more) 76 a.a.
Ligands
GOL ×9
SO4 ×6
Waters ×128
PDB id:
5af4
Name: Signaling protein
Title: Structure of lys33-linked diub
Structure: Ubiquitin. Chain: a, b, c, d, e, f, g, h. Synonym: polyubiquitin-c. Engineered: yes. Mutation: yes. Other_details: isopeptide linkages between a76 c and b33 nz, c76 c and d33 nz, e76 c and f33 nz
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: rosettaii.
Resolution:
1.85Å     R-factor:   0.233     R-free:   0.256
Authors: M.A.Michel,P.R.Elliott,K.N.Swatek,M.Simicek,J.N.Pruneda,J.L.Wagstaff, S.M.V.Freund,D.Komander
Key ref: M.A.Michel et al. (2015). Assembly and specific recognition of k29- and k33-linked polyubiquitin. Mol Cell, 58, 95. PubMed id: 25752577 DOI: 10.1016/j.molcel.2015.01.042
Date:
19-Jan-15     Release date:   25-Mar-15    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P0CG48  (UBC_HUMAN) -  Polyubiquitin-C from Homo sapiens
Seq:
Struc:
 
Seq:
Struc:
685 a.a.
76 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.?
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

 

 
DOI no: 10.1016/j.molcel.2015.01.042 Mol Cell 58:95 (2015)
PubMed id: 25752577  
 
 
Assembly and specific recognition of k29- and k33-linked polyubiquitin.
M.A.Michel, P.R.Elliott, K.N.Swatek, M.Simicek, J.N.Pruneda, J.L.Wagstaff, S.M.Freund, D.Komander.
 
  ABSTRACT  
 
No abstract given.

 

 

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