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PDBsum entry 5aem
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Transcription
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PDB id
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5aem
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References listed in PDB file
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Key reference
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Title
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Architecture of tfiiic and its role in RNA polymerase iii pre-Initiation complex assembly.
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Authors
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G.Male,
A.Von appen,
S.Glatt,
N.M.Taylor,
M.Cristovao,
H.Groetsch,
M.Beck,
C.W.Müller.
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Ref.
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Nat Commun, 2015,
6,
7387.
[DOI no: ]
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PubMed id
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Abstract
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In eukaryotes, RNA Polymerase III (Pol III) is specifically responsible for
transcribing genes encoding tRNAs and other short non-coding RNAs. The
recruitment of Pol III to tRNA-encoding genes requires the transcription factors
(TF) IIIB and IIIC. TFIIIC has been described as a conserved, multi-subunit
protein complex composed of two subcomplexes, called τA and τB. How these two
subcomplexes are linked and how their interaction affects the formation of the
Pol III pre-initiation complex (PIC) is poorly understood. Here we use chemical
crosslinking mass spectrometry and determine the molecular architecture of
TFIIIC. We further report the crystal structure of the essential TPR array from
τA subunit τ131 and characterize its interaction with a central region of τB
subunit τ138. The identified τ131-τ138 interacting region is essential in
vivo and overlaps with TFIIIB-binding sites, revealing a crucial interaction
platform for the regulation of tRNA transcription initiation.
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