PDBsum entry 5aem

Transcription

PDB id: 5aem

Contents

Protein chain

407 a.a.

PDB id:

5aem

Name:

Transcription

Title:

Structure of t131 n-terminal tpr array

Structure:

Transcription factor tau 131 kda subunit. Chain: a. Fragment: n-terminal tpr array, residues 123-566. Synonym: tfiiic 131 kda subunit, transcription factor c subunit 4, tau131. Engineered: yes

Source:

Saccharomyces cerevisiae s288c. Organism_taxid: 559292. Atcc: 204508. Expressed in: escherichia coli bl21. Expression_system_taxid: 511693. Expression_system_variant: star prare.

Resolution:

3.40Å R-factor: 0.211 R-free: 0.245

Authors:

N.M.I.Taylor,C.W.Muller

Key ref:

G.Male et al. (2015). Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly. Nat Commun, 6, 7387. PubMed id: 26060179 DOI: 10.1038/ncomms8387

Date:

05-Jan-15 Release date: 24-Jun-15

Protein chain

P33339  (TFC4_YEAST) -  Transcription factor tau 131 kDa subunit from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1025 a.a.

Struc: 407 a.a.

DOI no: 10.1038/ncomms8387

Nat Commun 6:7387 (2015)

PubMed id: 26060179

Architecture of TFIIIC and its role in RNA polymerase III pre-initiation complex assembly.

G.Male, A.von Appen, S.Glatt, N.M.Taylor, M.Cristovao, H.Groetsch, M.Beck, C.W.Müller.

ABSTRACT

In eukaryotes, RNA Polymerase III (Pol III) is specifically responsible for transcribing genes encoding tRNAs and other short non-coding RNAs. The recruitment of Pol III to tRNA-encoding genes requires the transcription factors (TF) IIIB and IIIC. TFIIIC has been described as a conserved, multi-subunit protein complex composed of two subcomplexes, called τA and τB. How these two subcomplexes are linked and how their interaction affects the formation of the Pol III pre-initiation complex (PIC) is poorly understood. Here we use chemical crosslinking mass spectrometry and determine the molecular architecture of TFIIIC. We further report the crystal structure of the essential TPR array from τA subunit τ131 and characterize its interaction with a central region of τB subunit τ138. The identified τ131-τ138 interacting region is essential in vivo and overlaps with TFIIIB-binding sites, revealing a crucial interaction platform for the regulation of tRNA transcription initiation.