PDBsum entry 5aek

Hydrolase

PDB id: 5aek

Contents

Protein chains

(+ 6 more) 223 a.a.

(+ 6 more) 78 a.a.

PDB id:

5aek

Name:

Hydrolase

Title:

Crystal structure of the human senp2 c548s in complex with the human sumo1 k48m f66w

Structure:

Sentrin-specific protease 2. Chain: a, c, e, g, i, k, m, o, q, s, u, w. Fragment: catalytic domain. Synonym: axam2, smt3-specific isopeptidase 2, smt3ip2, sentrin/sumo- specific protease senp2. Engineered: yes. Mutation: yes. Small ubiquitin-related modifier 1. Chain: b, d, f, h, j, l, n, p, r, t, v, x.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

3.00Å R-factor: 0.259 R-free: 0.326

Authors:

P.Gallego,R.Grana-Montes,A.Espargaro,V.Castillo,J.Torrent,R.Lange, E.Papaleo,K.Lindorff-Larsend,S.Ventura,D.Reverter

Key ref:

R.Grana-Montes et al. Stepping back and forward on sumo folding evolution. To be published, .

Date:

23-Dec-14 Release date: 20-Jan-16

Protein chains

Q9HC62  (SENP2_HUMAN) -  Sentrin-specific protease 2 from Homo sapiens

Seq: 589 a.a.

Struc: 223 a.a.*

Protein chains

P63165  (SUMO1_HUMAN) -  Small ubiquitin-related modifier 1 from Homo sapiens

Seq: 101 a.a.

Struc: 78 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class 2: Chains A, C, E, G, I, K, M, O, Q, S, U, W: E.C.3.4.22.- - ?????
• Enzyme class 3: Chains B, D, F, H, J, L, N, P, R, T, V, X: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.