PDBsum entry 5a7a

Virus

PDB id: 5a7a

Contents

Protein chain

175 a.a.

DNA/RNA

PDB id:

5a7a

Name:

Virus

Title:

Novel inter-subunit contacts in barley stripe mosaic virus revealed by cryo-em

Structure:

Barley stripe mosaic virus narrow. Chain: a. Engineered: yes. Other_details: only contains residues 3-131 and 148-193 in structure. RNA. Chain: r. Engineered: yes

Source:

Barley stripe mosaic virus. Organism_taxid: 12327. Strain: nd18. Atcc: pv-328. Expressed in: agrobacterium tumefaciens. Expression_system_taxid: 358. Other_details: purified from nicotiana benthamiana infected plants. Tobacco mosaic virus. Organism_taxid: 12242.

Authors:

D.K.Clare,E.Pechnikova,E.Skurat,V.Makarov,O.S.Sokolova,A.G.Solovyev, E.V Orlova

Key ref:

D.K.Clare et al. (2015). Novel Inter-Subunit Contacts in Barley Stripe Mosaic Virus Revealed by Cryo-Electron Microscopy. Structure, 23, 1815-1826. PubMed id: 26278173 DOI: 10.1016/j.str.2015.06.028

Date:

03-Jul-15 Release date: 02-Sep-15

Protein chain

P04866  (CAPSD_BSMV) -  Capsid protein from Barley stripe mosaic virus

Seq: 198 a.a.

Struc: 175 a.a.

DNA/RNA chain

G-A-A 3 bases

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.1016/j.str.2015.06.028

Structure 23:1815-1826 (2015)

PubMed id: 26278173

Novel Inter-Subunit Contacts in Barley Stripe Mosaic Virus Revealed by Cryo-Electron Microscopy.

D.K.Clare, E.V.Pechnikova, E.V.Skurat, V.V.Makarov, O.S.Sokolova, A.G.Solovyev, E.V.Orlova.

ABSTRACT

Barley stripe mosaic virus (BSMV, genus Hordeivirus) is a rod-shaped single-stranded RNA virus similar to viruses of the structurally characterized and well-studied genus Tobamovirus. Here we report the first high-resolution structure of BSMV at 4.1 Å obtained by cryo-electron microscopy. We discovered that BSMV forms two types of virion that differ in the number of coat protein (CP) subunits per turn and interactions between the CP subunits. While BSMV and tobacco mosaic virus CP subunits have a similar fold and interact with RNA using conserved residues, the axial contacts between the CP of these two viral groups are considerably different. BSMV CP subunits lack substantial axial contacts and are held together by a previously unobserved lateral contact formed at the virion surface via an interacting loop, which protrudes from the CP hydrophobic core to the adjacent CP subunit. These data provide an insight into diversity in structural organization of helical viruses.