|
|
||
|
|
|
|
|
UniProt functional annotation for P43593 | ||
|
|
|
|
|
|
||
| UniProt code: P43593. |
|
||
| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
|
||
|
||
| Function: | |
Predominant proteasome-associated deubiquitinase which releases ubiquitin from the proteasome targeted ubiquitinated proteins. Ensures the regeneration of ubiquitin at the proteasome. Has proteasome-inhibitory activity and delays the degradation of ubiquitinated proteins to provide a time window allowing gradual deubiquitination of the substrate. Stabilizes the association of HUL5 with proteasomes and works in opposition to polyubiquitin elongation activity of HUL5. {ECO:0000269|PubMed:10527495, ECO:0000269|PubMed:12408819, ECO:0000269|PubMed:14581483, ECO:0000269|PubMed:17018280, ECO:0000269|PubMed:17190603, ECO:0000269|PubMed:9344467}. |
|
||
| Catalytic activity: | |
Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76- residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; |
| Activity regulation: | |
Activated by it's association with the proteasome. {ECO:0000269|PubMed:12408819}. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 8.5-9. {ECO:0000269|PubMed:9344467}; |
| Subunit: | |
Associates with the regulatory particle (RP) of the proteasome. |
| Domain: | |
The N-terminal ubiquitin-like domain is required for proteasome association and UBP6 activation at the proteasome. {ECO:0000269|PubMed:10386876, ECO:0000269|PubMed:12408819}. |
| Miscellaneous: | |
Present with 6770 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the peptidase C19 family. USP14/UBP6 subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.