UniProt functional annotation for P53197



	UniProt functional annotation for P53197

UniProt code: P53197.

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).

Taxonomy: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.

Function: Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). During telophase and in the subsequent G1 phase of the cell cycle, recognizes and binds proteins containing a destruction box (D-box) and an additional degradation signal termed the KEN box including ASE1, CDC20, the B-type cyclins CLB2 and CLB3, the polo-like kinase CDC5 and HSL1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1. Probably is the target of a BUB2- dependent spindle checkpoint pathway. {ECO:0000269|PubMed:10329618, ECO:0000269|PubMed:11448992, ECO:0000269|PubMed:11566880}.

Subunit: Associates with the APC/C complex. Interacts with CLB2, CLB3, CDC5, HSL1, MSN5 and PSE1. {ECO:0000269|PubMed:11562348, ECO:0000269|PubMed:11566880, ECO:0000269|PubMed:12456658}.

Subcellular location: Cytoplasm {ECO:0000269|PubMed:12456658}. Nucleus {ECO:0000269|PubMed:12456658}. Note=Nuclear import and export are mediated by the importin PSE1 and the exportin MSN5.

Domain: The C-box is required for the association with the APC/C complex. {ECO:0000250}.

Ptm: Phosphorylated at multiple sites by CDC28, probably in its CLB5 bound form, in S, G2 and M phase of the cell cycle, thereby blocking the association of CDH1 to the APC/C and promoting nuclear export of CDH1 by MSN5. Dephosphorylated and activated by CDC14 in late anaphase, which may be necessary for PSE1-dependent nuclear localization. {ECO:0000269|PubMed:14574415, ECO:0000269|PubMed:9831566}.

Similarity: Belongs to the WD repeat CDC20/Fizzy family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Taxonomy:		Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.



Function:		Activator protein that regulates the ubiquitin ligase activity and substrate specificity of the anaphase promoting complex/cyclosome (APC/C). During telophase and in the subsequent G1 phase of the cell cycle, recognizes and binds proteins containing a destruction box (D-box) and an additional degradation signal termed the KEN box including ASE1, CDC20, the B-type cyclins CLB2 and CLB3, the polo-like kinase CDC5 and HSL1, and recruits them in a C-box-dependent manner to the APC/C for ubiquitination and subsequent proteolysis. Required for exit from mitosis, cytokinesis and formation of prereplicative complexes in G1. Probably is the target of a BUB2- dependent spindle checkpoint pathway. {ECO:0000269\|PubMed:10329618, ECO:0000269\|PubMed:11448992, ECO:0000269\|PubMed:11566880}.


Subunit:		Associates with the APC/C complex. Interacts with CLB2, CLB3, CDC5, HSL1, MSN5 and PSE1. {ECO:0000269\|PubMed:11562348, ECO:0000269\|PubMed:11566880, ECO:0000269\|PubMed:12456658}.
Subcellular location:		Cytoplasm {ECO:0000269\|PubMed:12456658}. Nucleus {ECO:0000269\|PubMed:12456658}. Note=Nuclear import and export are mediated by the importin PSE1 and the exportin MSN5.
Domain:		The C-box is required for the association with the APC/C complex. {ECO:0000250}.
Ptm:		Phosphorylated at multiple sites by CDC28, probably in its CLB5 bound form, in S, G2 and M phase of the cell cycle, thereby blocking the association of CDH1 to the APC/C and promoting nuclear export of CDH1 by MSN5. Dephosphorylated and activated by CDC14 in late anaphase, which may be necessary for PSE1-dependent nuclear localization. {ECO:0000269\|PubMed:14574415, ECO:0000269\|PubMed:9831566}.
Similarity:		Belongs to the WD repeat CDC20/Fizzy family. {ECO:0000305}.