PDBsum entry 5a1x

Transport protein

PDB id: 5a1x

Contents

Protein chains

159 a.a.

813 a.a.

803 a.a.

550 a.a.

139 a.a.

813 a.a.

380 a.a.

824 a.a.

245 a.a.

PDB id:

5a1x

Name:

Transport protein

Title:

The structure of the copi coat linkage iii

Structure:

Adp-ribosylation factor 1. Chain: a, b, i, j. Engineered: yes. Coatomer subunit alpha. Chain: c, k. Synonym: alpha-coat protein, alpha-cop, xenopsin-related peptide. Engineered: yes. Coatomer subunit beta'. Chain: d, l.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Expressed in: escherichia coli. Expression_system_taxid: 469008. Mus musculus. House mouse. Organism_taxid: 10090. Expressed in: spodoptera frugiperda.

Authors:

S.O.Dodonova,P.Diestelkoetter-Bachert,A.Von Appen,W.J.H.Hagen,R.Beck, M.Beck,F.Wieland,J.A.G.Briggs

Key ref:

S.O.Dodonova et al. (2015). VESICULAR TRANSPORT. A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly. Science, 349, 195-198. PubMed id: 26160949 DOI: 10.1126/science.aab1121

Date:

06-May-15 Release date: 08-Jul-15

Protein chains

P11076  (ARF1_YEAST) -  ADP-ribosylation factor 1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 181 a.a.

Struc: 159 a.a.

Protein chains

Q8CIE6  (COPA_MOUSE) -  Coatomer subunit alpha from Mus musculus

Seq: 1224 a.a.

Struc: 813 a.a.

Protein chains

O55029  (COPB2_MOUSE) -  Coatomer subunit beta' from Mus musculus

Seq: 905 a.a.

Struc: 803 a.a.

Protein chain

Q9QZE5  (COPG1_MOUSE) -  Coatomer subunit gamma-1 from Mus musculus

Seq: 874 a.a.

Struc: 550 a.a.

Protein chains

P61924  (COPZ1_MOUSE) -  Coatomer subunit zeta-1 from Mus musculus

Seq: 177 a.a.

Struc: 139 a.a.

Protein chains

Q9JIF7  (COPB_MOUSE) -  Coatomer subunit beta from Mus musculus

Seq: 953 a.a.

Struc: 813 a.a.

Protein chains

Q5XJY5  (COPD_MOUSE) -  Coatomer subunit delta from Mus musculus

Seq: 511 a.a.

Struc: 380 a.a.

Protein chain

Q9QZE5  (COPG1_MOUSE) -  Coatomer subunit gamma-1 from Mus musculus

Seq: 874 a.a.

Struc: 824 a.a.

Protein chain

Q5XJY5  (COPD_MOUSE) -  Coatomer subunit delta from Mus musculus

Seq: 511 a.a.

Struc: 245 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, I, J: E.C.3.6.5.2 - small monomeric GTPase.
• Reaction: GTP + H2O = GDP + phosphate + H⁺

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

DOI no: 10.1126/science.aab1121

Science 349:195-198 (2015)

PubMed id: 26160949

VESICULAR TRANSPORT. A structure of the COPI coat and the role of coat proteins in membrane vesicle assembly.

S.O.Dodonova, P.Diestelkoetter-Bachert, A.von Appen, W.J.Hagen, R.Beck, M.Beck, F.Wieland, J.A.Briggs.

ABSTRACT

Transport of material within cells is mediated by trafficking vesicles that bud from one cellular compartment and fuse with another. Formation of a trafficking vesicle is driven by membrane coats that localize cargo and polymerize into cages to bend the membrane. Although extensive structural information is available for components of these coats, the heterogeneity of trafficking vesicles has prevented an understanding of how complete membrane coats assemble on the membrane. We combined cryo-electron tomography, subtomogram averaging, and cross-linking mass spectrometry to derive a complete model of the assembled coat protein complex I (COPI) coat involved in traffic between the Golgi and the endoplasmic reticulum. The highly interconnected COPI coat structure contradicted the current "adaptor-and-cage" understanding of coated vesicle formation.