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PDBsum entry 5a1v
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Transport protein
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PDB id
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5a1v
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Contents |
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(+ 0 more)
159 a.a.
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813 a.a.
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803 a.a.
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824 a.a.
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139 a.a.
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813 a.a.
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135 a.a.
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1126 a.a.
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292 a.a.
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References listed in PDB file
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Key reference
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Title
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Vesicular transport. A structure of the copi coat and the role of coat proteins in membrane vesicle assembly.
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Authors
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S.O.Dodonova,
P.Diestelkoetter-Bachert,
A.Von appen,
W.J.Hagen,
R.Beck,
M.Beck,
F.Wieland,
J.A.Briggs.
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Ref.
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Science, 2015,
349,
195-198.
[DOI no: ]
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PubMed id
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Abstract
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Transport of material within cells is mediated by trafficking vesicles that bud
from one cellular compartment and fuse with another. Formation of a trafficking
vesicle is driven by membrane coats that localize cargo and polymerize into
cages to bend the membrane. Although extensive structural information is
available for components of these coats, the heterogeneity of trafficking
vesicles has prevented an understanding of how complete membrane coats assemble
on the membrane. We combined cryo-electron tomography, subtomogram averaging,
and cross-linking mass spectrometry to derive a complete model of the assembled
coat protein complex I (COPI) coat involved in traffic between the Golgi and the
endoplasmic reticulum. The highly interconnected COPI coat structure
contradicted the current "adaptor-and-cage" understanding of coated
vesicle formation.
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