PDBsum entry 5wjh

Hydrolase

PDB id: 5wjh

Contents

Protein chain

279 a.a.

Ligands

MHA

Metals

_CA

Waters ×479

PDB id:

5wjh

Name:

Hydrolase

Title:

Using sound pulses to solve the crystal harvesting bottleneck

Structure:

Proteinase k. Chain: a. Synonym: endopeptidase k,tritirachium alkaline proteinase. Ec: 3.4.21.64

Source:

Parengyodontium album. Organism_taxid: 37998

Resolution:

1.63Å R-factor: 0.191 R-free: 0.249

Authors:

A.S.Soares,H.M.Brennan,L.Mccarthy,L.Leroy

Key ref:

Y.N.Samara et al. (2018). Using sound pulses to solve the crystal-harvesting bottleneck. Acta Crystallogr D Struct Biol, 74, 986-999. PubMed id: 30289409

Date:

22-Jul-17 Release date: 02-Aug-17

Protein chain

P06873  (PRTK_PARAQ) -  Proteinase K from Parengyodontium album

Seq: 384 a.a.

Struc: 279 a.a.*

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: E.C.3.4.21.64 - peptidase K.
• Reaction: Hydrolysis of keratin and of other proteins, with subtilisin-like specificity. Hydrolyzes peptides amides.

Acta Crystallogr D Struct Biol 74:986-999 (2018)

PubMed id: 30289409

Using sound pulses to solve the crystal-harvesting bottleneck.

Y.N.Samara, H.M.Brennan, L.McCarthy, M.T.Bollard, D.Laspina, J.M.Wlodek, S.L.Campos, R.Natarajan, K.Gofron, S.McSweeney, A.S.Soares, L.Leroy.

ABSTRACT

Crystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acoustically harvested from slightly modified MiTeGen In Situ-1 crystallization plates. This technique uses an acoustic pulse to eject each crystal out of its crystallization well, through a short air column and onto a micro-mesh (improving on previous work, which required separately grown crystals to be transferred before harvesting). Crystals can be individually harvested or can be serially combined with a chemical library such as a fragment library.