PDBsum entry 5k7s

Hydrolase

PDB id: 5k7s

Contents

Protein chain

279 a.a.

Metals

_CA ×2

Waters ×221

PDB id:

5k7s

Name:

Hydrolase

Title:

Microed structure of proteinase k at 1.6 a resolution

Structure:

Proteinase k. Chain: a. Synonym: endopeptidase k, tritirachium alkaline proteinase. Ec: 3.4.21.64

Source:

Engyodontium album. Organism_taxid: 37998

Authors:

M.J.De La Cruz,J.Hattne,D.Shi,P.Seidler,J.Rodriguez,F.E.Reyes, M.R.Sawaya,D.Cascio,D.Eisenberg,T.Gonen

Key ref:

M.J.de la Cruz et al. (2017). Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED. Nat Methods, 14, 399-402. PubMed id: 28192420

Date:

26-May-16 Release date: 05-Apr-17

Protein chain

P06873  (PRTK_PARAQ) -  Proteinase K from Parengyodontium album

Seq: 384 a.a.

Struc: 279 a.a.

Enzyme reactions

• Enzyme class: E.C.3.4.21.64 - peptidase K.
• Reaction: Hydrolysis of keratin and of other proteins, with subtilisin-like specificity. Hydrolyzes peptides amides.

Nat Methods 14:399-402 (2017)

PubMed id: 28192420

Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED.

M.J.de la Cruz, J.Hattne, D.Shi, P.Seidler, J.Rodriguez, F.E.Reyes, M.R.Sawaya, D.Cascio, S.C.Weiss, S.K.Kim, C.S.Hinck, A.P.Hinck, G.Calero, D.Eisenberg, T.Gonen.

ABSTRACT

Traditionally, crystallographic analysis of macromolecules has depended on large, well-ordered crystals, which often require significant effort to obtain. Even sizable crystals sometimes suffer from pathologies that render them inappropriate for high-resolution structure determination. Here we show that fragmentation of large, imperfect crystals into microcrystals or nanocrystals can provide a simple path for high-resolution structure determination by the cryoEM method MicroED and potentially by serial femtosecond crystallography.