PDBsum entry 5hm2

Hydrolase

PDB id: 5hm2

Contents

Protein chains

201 a.a.

PDB id:

5hm2

Name:

Hydrolase

Title:

Crystal structure of the 3c protease from south african territories type 2 foot-and-mouth disease virus

Structure:

3c proteinase. Chain: a, b, c, d, e. Engineered: yes. Mutation: yes. Other_details: gly 1 is a vec

Source:

Foot-and-mouth disease virus - type sat 2. Organism_taxid: 35292. Strain: sat2/gha/8/91. Gene: 3c. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

3.20Å R-factor: 0.225 R-free: 0.272

Authors:

J.Yang,E.N.Leen,S.Curry

Key ref:

J.Yang et al. (2016). Crystal structure of the 3C protease from Southern African Territories type 2 foot-and-mouth disease virus. Peerj, 4, e1964. PubMed id: 27168976 DOI: 10.7717/peerj.1964

Date:

15-Jan-16 Release date: 02-Mar-16

Protein chains

X5CZS0  (X5CZS0_9PICO) -  p2/P3 polyprotein (Fragment) from Foot-and-mouth disease virus SAT 2

Seq: 1396 a.a.

Struc: 201 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.?

DOI no: 10.7717/peerj.1964

Peerj 4:e1964 (2016)

PubMed id: 27168976

Crystal structure of the 3C protease from Southern African Territories type 2 foot-and-mouth disease virus.

J.Yang, E.N.Leen, F.F.Maree, S.Curry.

ABSTRACT

The replication of foot-and-mouth disease virus (FMDV) is dependent on the virus-encoded 3C protease (3C(pro)). As in other picornaviruses, 3C(pro) performs most of the proteolytic processing of the polyprotein expressed from the large open reading frame in the RNA genome of the virus. Previous work revealed that the 3C(pro) from serotype A-one of the seven serotypes of FMDV-adopts a trypsin-like fold. On the basis of capsid sequence comparisons the FMDV serotypes are grouped into two phylogenetic clusters, with O, A, C, and Asia 1 in one, and the three Southern African Territories serotypes, (SAT-1, SAT-2 and SAT-3) in another, a grouping pattern that is broadly, but not rigidly, reflected in 3C(pro) amino acid sequences. We report here the cloning, expression and purification of 3C proteases from four SAT serotype viruses (SAT2/GHA/8/91, SAT1/NIG/5/81, SAT1/UGA/1/97, and SAT2/ZIM/7/83) and the crystal structure at 3.2 Å resolution of 3C(pro) from SAT2/GHA/8/91.