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PDBsum entry 5fw1

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protein dna_rna metals links
Hydrolase/DNA PDB id
5fw1
Jmol PyMol
Contents
Protein chain
1304 a.a.
DNA/RNA
Metals
_MG ×2
__K ×13
Waters ×301
PDB id:
5fw1
Name: Hydrolase/DNA
Title: Crystal structure of spycas9 variant vqr bound to sgrna and tgag pam target DNA
Structure: Crispr-associated endonuclease cas9/csn1. Chain: b. Synonym: spycas9. Engineered: yes. Mutation: yes. Sgrna. Chain: a. Target DNA strand. Chain: c.
Source: Streptococcus pyogenes. Organism_taxid: 1314. Strain: m1. Expressed in: escherichia coli. Expression_system_taxid: 562. Expression_system_variant: rosetta 2. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.
Resolution:
2.50Å     R-factor:   0.220     R-free:   0.254
Authors: C.Anders,K.Bargsten,M.Jinek
Date:
11-Feb-16     Release date:   20-Apr-16    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q99ZW2  (Q99ZW2_STRP1) -  CRISPR-associated endonuclease Cas9/Csn1
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1368 a.a.
1304 a.a.*
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 6 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.1.-.-
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     nucleic acid phosphodiester bond hydrolysis   3 terms 
  Biochemical function     hydrolase activity     9 terms  

 

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