PDBsum entry 5dae

Protein binding

PDB id

5dae

Loading ...

Contents

			Protein chains

					57 a.a.


					53 a.a.

			Waters ×89

PDB id:

5dae

Links

Name:

Protein binding

Title:

Kazal type inhibitor from salivary glands of aedes aegypti mosquito

Structure:

Aael006007-pa. Chain: a, b. Fragment: unp residues 27-91. Synonym: kazal domain-containing peptide. Engineered: yes

Source:

Aedes aegypti. Yellowfever mosquito. Organism_taxid: 7159. Gene: aael006007. Expressed in: komagataella pastoris gs115. Expression_system_taxid: 644223.

Resolution:

1.40Å

R-factor:

0.183

R-free:

0.230

Authors:

R.J.S.Torquato,P.J.B.Pereira,A.S.Tanaka

Key ref:

R.J.S.Torquato et al. (2017). High-resolution structure of a Kazal-type serine protease inhibitor from the dengue vector Aedes aegypti. Acta Crystallogr F Struct Biol Commun, 73, 469-475. PubMed id: 28777090 DOI: 10.1107/S2053230X17010007

Date:

19-Aug-15

Release date:

07-Sep-16

PROCHECK

	Headers

	References

Protein chain

Q1HRB8 (Q1HRB8_AEDAE) - Kazal domain-containing peptide from Aedes aegypti

Seq: Struc:					91 a.a. 57 a.a.

Protein chain

Q1HRB8 (Q1HRB8_AEDAE) - Kazal domain-containing peptide from Aedes aegypti

Seq: Struc:					91 a.a. 53 a.a.

Key:

PfamA domain

Secondary structure

CATH domain

DOI no: 10.1107/S2053230X17010007	Acta Crystallogr F Struct Biol Commun 73:469-475 (2017)
PubMed id: 28777090

High-resolution structure of a Kazal-type serine protease inhibitor from the dengue vector Aedes aegypti.
R.J.S.Torquato, S.Lu, N.H.Martins, A.S.Tanaka, P.J.B.Pereira.

ABSTRACT

Blood-feeding exoparasites are rich sources of protease inhibitors, and the mosquito Aedes aegypti, which is a vector of Dengue virus, Yellow fever virus, Chikungunya virus and Zika virus, is no exception. AaTI is a single-domain, noncanonical Kazal-type serine proteinase inhibitor from A. aegypti that recognizes both digestive trypsin-like serine proteinases and the central protease in blood clotting, thrombin, albeit with an affinity that is three orders of magnitude lower. Here, the 1.4 Å resolution crystal structure of AaTI is reported from extremely tightly packed crystals (∼22% solvent content), revealing the structural determinants for the observed inhibitory profile of this molecule.