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UniProt functional annotation for Q97VW7 | ||
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| UniProt code: Q97VW7. |
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| Organism: | |
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus). |
| Taxonomy: | |
Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Saccharolobus. |
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| Function: | |
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. {ECO:0000255|HAMAP-Rule:MF_00772}. |
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| Catalytic activity: | |
Reaction=a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = a 2'-deoxyguanosine in DNA + S-methyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:24000, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:10132, Rhea:RHEA-COMP:11367, Rhea:RHEA-COMP:11368, ChEBI:CHEBI:29950, ChEBI:CHEBI:82612, ChEBI:CHEBI:85445, ChEBI:CHEBI:85448; EC=2.1.1.63; Evidence={ECO:0000255|HAMAP-Rule:MF_00772}; |
| Catalytic activity: | |
Reaction=a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:53428, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:10132, Rhea:RHEA-COMP:13555, Rhea:RHEA-COMP:13556, ChEBI:CHEBI:29950, ChEBI:CHEBI:82612, ChEBI:CHEBI:137386, ChEBI:CHEBI:137387; EC=2.1.1.63; Evidence={ECO:0000255|HAMAP-Rule:MF_00772}; |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00772}. |
| Miscellaneous: | |
This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles. |
| Similarity: | |
Belongs to the MGMT family. {ECO:0000255|HAMAP- Rule:MF_00772}. |
Annotations taken from UniProtKB at the EBI.