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UniProt functional annotation for P28321 | ||
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| UniProt code: P28321. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Converts monoacylglycerides (MAG) to free fatty acids and glycerol (PubMed:20554061). Has a strong preference for monounsaturated monoglycerides (PubMed:26869448). Required for efficient degradation of MAG, short-lived intermediates of glycerolipid metabolism which may also function as lipid signaling molecules. Controls inactivation of the signaling lipid N-palmitoylethanolamine (PEA) (PubMed:19529773). Involved in fatty acid ethyl ester (FAEE) catabolism. FAEEs are non- oxidative metabolites of ethanol that are transiently incorporated into lipid droplets (LDs). Their mobilization by LD-resident FAEE hydrolases facilitates a controlled metabolism of these potentially toxic lipid metabolites (PubMed:27036938). {ECO:0000269|PubMed:19529773, ECO:0000269|PubMed:20554061, ECO:0000269|PubMed:26869448, ECO:0000269|PubMed:27036938}. |
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| Catalytic activity: | |
Reaction=Hydrolyzes glycerol monoesters of long-chain fatty acids.; EC=3.1.1.23; Evidence={ECO:0000269|PubMed:20554061}; |
| Catalytic activity: | |
Reaction=a fatty acid ethyl ester + H2O = a fatty acid + ethanol + H(+); Xref=Rhea:RHEA:50148, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16236, ChEBI:CHEBI:28868, ChEBI:CHEBI:78206; Evidence={ECO:0000269|PubMed:27036938}; |
| Catalytic activity: | |
Reaction=1-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + glycerol + H(+); Xref=Rhea:RHEA:38487, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, ChEBI:CHEBI:75342; Evidence={ECO:0000269|PubMed:20554061, ECO:0000269|PubMed:26869448}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38488; Evidence={ECO:0000305|PubMed:20554061}; |
| Catalytic activity: | |
Reaction=2-(9Z-octadecenoyl)-glycerol + H2O = (9Z)-octadecenoate + glycerol + H(+); Xref=Rhea:RHEA:38491, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:30823, ChEBI:CHEBI:73990; Evidence={ECO:0000269|PubMed:20554061}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:38492; Evidence={ECO:0000305|PubMed:20554061}; |
| Catalytic activity: | |
Reaction=1-hexadecanoylglycerol + H2O = glycerol + H(+) + hexadecanoate; Xref=Rhea:RHEA:39959, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:69081; Evidence={ECO:0000269|PubMed:20554061, ECO:0000269|PubMed:27036938}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39960; Evidence={ECO:0000305|PubMed:20554061, ECO:0000305|PubMed:27036938}; |
| Catalytic activity: | |
Reaction=2-hexadecanoylglycerol + H2O = glycerol + H(+) + hexadecanoate; Xref=Rhea:RHEA:39963, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17754, ChEBI:CHEBI:75455; Evidence={ECO:0000269|PubMed:20554061}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:39964; Evidence={ECO:0000305|PubMed:20554061}; |
| Catalytic activity: | |
Reaction=ethyl hexadecanoate + H2O = ethanol + H(+) + hexadecanoate; Xref=Rhea:RHEA:50132, ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16236, ChEBI:CHEBI:84932; Evidence={ECO:0000269|PubMed:27036938}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50133; Evidence={ECO:0000305|PubMed:27036938}; |
| Catalytic activity: | |
Reaction=ethyl (9Z)-octadecenoate + H2O = (9Z)-octadecenoate + ethanol + H(+); Xref=Rhea:RHEA:50136, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16236, ChEBI:CHEBI:30823, ChEBI:CHEBI:84940; Evidence={ECO:0000269|PubMed:27036938}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50137; Evidence={ECO:0000305|PubMed:27036938}; |
| Catalytic activity: | |
Reaction=ethyl (9Z)-hexadecenoate + H2O = (9Z)-hexadecenoate + ethanol + H(+); Xref=Rhea:RHEA:50144, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16236, ChEBI:CHEBI:32372, ChEBI:CHEBI:84934; Evidence={ECO:0000269|PubMed:27036938}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50145; Evidence={ECO:0000305|PubMed:27036938}; |
| Catalytic activity: | |
Reaction=ethyl octadecanoate + H2O = ethanol + H(+) + octadecanoate; Xref=Rhea:RHEA:50316, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16236, ChEBI:CHEBI:25629, ChEBI:CHEBI:84936; Evidence={ECO:0000269|PubMed:27036938}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50317; Evidence={ECO:0000305|PubMed:27036938}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=260 uM for rac-1(3)-oleoylglycerol {ECO:0000269|PubMed:20554061}; Vmax=10.3 mmol/h/mg enzyme {ECO:0000269|PubMed:20554061}; pH dependence: Active from pH 4.5 to 8. {ECO:0000269|PubMed:20554061}; |
| Pathway: | |
Glycerolipid metabolism; triacylglycerol degradation. {ECO:0000269|PubMed:20554061}. |
| Subcellular location: | |
Lipid droplet {ECO:0000269|PubMed:10515935, ECO:0000269|PubMed:20554061, ECO:0000269|PubMed:21820081, ECO:0000269|PubMed:24868093}. Cytoplasm {ECO:0000269|PubMed:11914276}. Endoplasmic reticulum {ECO:0000269|PubMed:14562095}. Mitochondrion outer membrane {ECO:0000269|PubMed:16407407}. Note=Although the protein is identified in the cytoplasm, several membrane systems and lipid droplets, MGL activity is only measured in membrane fractions and lipid droplets. {ECO:0000269|PubMed:20554061}. |
| Miscellaneous: | |
Present with 2140 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}. |
| Similarity: | |
Belongs to the AB hydrolase superfamily. Monoacylglycerol lipase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.