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UniProt functional annotation for R4JK63 | ||
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| UniProt code: R4JK63. |
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| Organism: | |
Porcine epidemic diarrhea virus. |
| Taxonomy: | |
Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Pedacovirus. |
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| Function: | |
NendoU is a Mn(2+)-dependent, uridylate-specific enzyme, which leaves 2'-3'-cyclic phosphates 5' to the cleaved bond. {ECO:0000256|ARBA:ARBA00002136}. |
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| Function: | |
Nsp7-nsp8 hexadecamer may possibly confer processivity to the polymerase, maybe by binding to dsRNA or by producing primers utilized by the latter. {ECO:0000256|ARBA:ARBA00002928}. |
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| Function: | |
Nsp9 is a ssRNA-binding protein. {ECO:0000256|ARBA:ARBA00003140}. |
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| Function: | |
The exoribonuclease acts on both ssRNA and dsRNA in a 3' to 5' direction. {ECO:0000256|ARBA:ARBA00003315}. |
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| Function: | |
The papain-like proteinase 1 (PLP1) and papain-like proteinase 2 (PLP2) are responsible for the cleavages located at the N- terminus of the replicase polyprotein. In addition, PLP2 possesses a deubiquitinating/deISGylating activity and processes both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains from cellular substrates. PLP2 also antagonizes innate immune induction of type I interferon by blocking the nuclear translocation of host IRF-3. {ECO:0000256|ARBA:ARBA00003396}. |
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| Function: | |
The replicase polyprotein of coronaviruses is a multifunctional protein: it contains the activities necessary for the transcription of negative stranded RNA, leader RNA, subgenomic mRNAs and progeny virion RNA as well as proteinases responsible for the cleavage of the polyprotein into functional products. {ECO:0000256|ARBA:ARBA00003368}. |
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| Catalytic activity: | |
Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.12; Evidence={ECO:0000256|ARBA:ARBA00001665}; |
| Catalytic activity: | |
Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13; Evidence={ECO:0000256|ARBA:ARBA00001556}; |
| Catalytic activity: | |
Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76- residue protein attached to proteins as an intracellular targeting signal).; EC=3.4.19.12; Evidence={ECO:0000256|ARBA:ARBA00000707}; |
| Subunit: | |
3CL-PRO exists as monomer and homodimer. Eight copies of nsp7 and eight copies of nsp8 assemble to form a heterohexadecamer. Nsp9 is a dimer. Nsp10 forms a dodecamer. {ECO:0000256|ARBA:ARBA00011639}. |
| Subcellular location: | |
Endoplasmic reticulum-Golgi intermediate compartment {ECO:0000256|ARBA:ARBA00004399}. Host cytoplasm, host perinuclear region {ECO:0000256|ARBA:ARBA00004407}. Host endoplasmic reticulum-Golgi intermediate compartment {ECO:0000256|ARBA:ARBA00004452}. Host membrane {ECO:0000256|ARBA:ARBA00004301}; Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004301}. Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. |
| Similarity: | |
Belongs to the coronaviruses polyprotein 1ab family. {ECO:0000256|ARBA:ARBA00008087}. |
Annotations taken from UniProtKB at the EBI.