UniProt functional annotation for P26993



	UniProt functional annotation for P26993

UniProt code: P26993.

Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas.

Function: Transcriptional regulator that plays an essential role in the activation the type III secretion system (T3SS) operons (PubMed:1655713, PubMed:26317977). In addition, ExsA directly regulates the transcription of ImpA virulence factor that cooperatively inhibits the functions of host macrophages together with the T3SS (PubMed:31527124). {ECO:0000269|PubMed:1655713, ECO:0000269|PubMed:26317977, ECO:0000269|PubMed:31527124}.

Activity regulation: In the absence of inducing signals such as low Ca(2+) or host cell contact, the T3SS/injectisome is expressed at a low basal level and exists in a quiescent state due to ExsA sequestration by ExsD in a 1:1 complex (PubMed:19369699, PubMed:20008065). Upon host cell contact, this interaction is disrupted by the anti-antiactivator protein ExsC leading to ExsA activation (PubMed:15225323). {ECO:0000269|PubMed:15225323, ECO:0000269|PubMed:19369699, ECO:0000269|PubMed:20008065}.

Subunit: Homodimer (PubMed:26317977). Interacts with ExsD; this interaction inhibits ExsA activity (PubMed:19369699, PubMed:20008065). {ECO:0000269|PubMed:19369699, ECO:0000269|PubMed:20008065, ECO:0000269|PubMed:26317977}.

Induction: By cAMP-dependent DNA-binding protein Vfr that directly activates exsA transcription from a promoter located immediately upstream of exsA. {ECO:0000269|PubMed:26929300}.

Disruption phenotype: Deletion mutants are defective in expression and secretion of type III secretion system-related exoproducts. {ECO:0000269|PubMed:15225323}.

Sequence caution: Sequence=AAA25816.1; Type=Erroneous initiation; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas.



Function:		Transcriptional regulator that plays an essential role in the activation the type III secretion system (T3SS) operons (PubMed:1655713, PubMed:26317977). In addition, ExsA directly regulates the transcription of ImpA virulence factor that cooperatively inhibits the functions of host macrophages together with the T3SS (PubMed:31527124). {ECO:0000269\|PubMed:1655713, ECO:0000269\|PubMed:26317977, ECO:0000269\|PubMed:31527124}.


Activity regulation:		In the absence of inducing signals such as low Ca(2+) or host cell contact, the T3SS/injectisome is expressed at a low basal level and exists in a quiescent state due to ExsA sequestration by ExsD in a 1:1 complex (PubMed:19369699, PubMed:20008065). Upon host cell contact, this interaction is disrupted by the anti-antiactivator protein ExsC leading to ExsA activation (PubMed:15225323). {ECO:0000269\|PubMed:15225323, ECO:0000269\|PubMed:19369699, ECO:0000269\|PubMed:20008065}.
Subunit:		Homodimer (PubMed:26317977). Interacts with ExsD; this interaction inhibits ExsA activity (PubMed:19369699, PubMed:20008065). {ECO:0000269\|PubMed:19369699, ECO:0000269\|PubMed:20008065, ECO:0000269\|PubMed:26317977}.
Induction:		By cAMP-dependent DNA-binding protein Vfr that directly activates exsA transcription from a promoter located immediately upstream of exsA. {ECO:0000269\|PubMed:26929300}.
Disruption phenotype:		Deletion mutants are defective in expression and secretion of type III secretion system-related exoproducts. {ECO:0000269\|PubMed:15225323}.
Sequence caution:		Sequence=AAA25816.1; Type=Erroneous initiation; Evidence={ECO:0000305};