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PDBsum entry 4zp0
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Transport protein
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PDB id
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4zp0
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References listed in PDB file
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Key reference
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Title
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Substrate-Bound structure of the e. Coli multidrug resistance transporter mdfa.
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Authors
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J.Heng,
Y.Zhao,
M.Liu,
Y.Liu,
J.Fan,
X.Wang,
Y.Zhao,
X.C.Zhang.
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Ref.
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Cell Res, 2015,
25,
1060-1073.
[DOI no: ]
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PubMed id
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Abstract
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Multidrug resistance is a serious threat to public health. Proton motive
force-driven antiporters from the major facilitator superfamily (MFS) constitute
a major group of multidrug-resistance transporters. Currently, no reports on
crystal structures of MFS antiporters in complex with their substrates exist.
The E. coli MdfA transporter is a well-studied model system for biochemical
analyses of multidrug-resistance MFS antiporters. Here, we report three crystal
structures of MdfA-ligand complexes at resolutions up to 2.0 Å, all in the
inward-facing conformation. The substrate-binding site sits proximal to the
conserved acidic residue, D34. Our mutagenesis studies support the structural
observations of the substrate-binding mode and the notion that D34 responds to
substrate binding by adjusting its protonation status. Taken together, our data
unveil the substrate-binding mode of MFS antiporters and suggest a mechanism of
transport via this group of transporters.
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