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PDBsum entry 4zni
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Viral protein
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PDB id
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4zni
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References listed in PDB file
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Key reference
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Title
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Structure and mechanism of the atpase that powers viral genome packaging.
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Authors
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B.J.Hilbert,
J.A.Hayes,
N.P.Stone,
C.M.Duffy,
B.Sankaran,
B.A.Kelch.
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Ref.
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Proc Natl Acad Sci U S A, 2015,
112,
E3792.
[DOI no: ]
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PubMed id
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Abstract
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Many viruses package their genomes into procapsids using an ATPase machine that
is among the most powerful known biological motors. However, how this motor
couples ATP hydrolysis to DNA translocation is still unknown. Here, we introduce
a model system with unique properties for studying motor structure and
mechanism. We describe crystal structures of the packaging motor ATPase domain
that exhibit nucleotide-dependent conformational changes involving a large
rotation of an entire subdomain. We also identify the arginine finger residue
that catalyzes ATP hydrolysis in a neighboring motor subunit, illustrating that
previous models for motor structure need revision. Our findings allow us to
derive a structural model for the motor ring, which we validate using
small-angle X-ray scattering and comparisons with previously published data. We
illustrate the model's predictive power by identifying the motor's DNA-binding
and assembly motifs. Finally, we integrate our results to propose a mechanistic
model for DNA translocation by this molecular machine.
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