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PDBsum entry 4zkb
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Viral protein/cytokine
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PDB id
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4zkb
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References listed in PDB file
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Key reference
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Title
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Structures of orf virus chemokine binding protein in complex with host chemokines reveal clues to broad binding specificity.
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Authors
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R.M.Couñago,
K.M.Knapp,
Y.Nakatani,
S.B.Fleming,
M.Corbett,
L.M.Wise,
A.A.Mercer,
K.L.Krause.
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Ref.
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Structure, 2015,
23,
1199-1213.
[DOI no: ]
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PubMed id
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Abstract
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The chemokine binding protein (CKBP) from orf virus (ORFV) binds with high
affinity to chemokines from three classes, C, CC, and CXC, making it unique
among poxvirus CKBPs described to date. We present its crystal structure alone
and in complex with three CC chemokines, CCL2, CCL3, and CCL7. ORFV CKBP
possesses a β-sandwich fold that is electrostatically and sterically
complementary to its binding partners. Chemokines bind primarily through
interactions involving the N-terminal loop and a hydrophobic recess on the ORFV
CKBP β-sheet II surface, and largely polar interactions between the chemokine
20s loop and a negatively charged surface groove located at one end of the CKBP
β-sheet II surface. ORFV CKBP interacts with leukocyte receptor and
glycosaminoglycan binding sites found on the surface of bound chemokines.
SEC-MALLS and chromatographic evidence is presented supporting that ORFV CKBP is
a dimer in solution over a broad range of protein concentrations.
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