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PDBsum entry 4zcj
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Viral protein
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PDB id
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4zcj
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References listed in PDB file
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Key reference
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Title
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Design and structure of an engineered disulfide-Stabilized influenza virus hemagglutinin trimer.
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Authors
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P.S.Lee,
X.Zhu,
W.Yu,
I.A.Wilson.
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Ref.
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J Virol, 2015,
89,
7417-7420.
[DOI no: ]
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PubMed id
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Abstract
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We engineered a disulfide-stabilized influenza virus hemagglutinin (HA) trimer,
termed HA3-SS, by introducing cysteine residues into the HA stem to covalently
bridge the three protomers. HA3-SS has increased thermostability compared to
wild-type HA, and binding of head- and stem-targeted antibodies (Abs) is
preserved; only minor structural changes are found in the vicinity of the
additional disulfide. This platform has been applied to H1 and H3 HAs and
provides prospects for design of intact, stabilized influenza virus HA
immunogens.
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