PDBsum entry 4z5t

Structural protein/DNA

PDB id: 4z5t

Contents

Protein chains

97 a.a.

83 a.a.

105 a.a.

92 a.a.

79 a.a.

DNA/RNA

PDB id:

4z5t

Name:

Structural protein/DNA

Title:

The nucleosome containing human h3.5

Structure:

Histone h3.3c. Chain: a, e. Synonym: histone h3.5. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes. Histone h2a type 1-b/e. Chain: c, g.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: h3f3c. Expressed in: escherichia coli. Expression_system_taxid: 469008. Gene: hist1h4a, h4/a, h4fa, hist1h4b, h4/i, h4fi, hist1h4c, h4/g, h4fg, hist1h4d, h4/b, h4fb, hist1h4e, h4/j, h4fj, hist1h4f, h4/c, h4fc, hist1h4h, h4/h, h4fh, hist1h4i, h4/m, h4fm, hist1h4j, h4/e,

Resolution:

2.80Å R-factor: 0.230 R-free: 0.269

Authors:

T.Urahama,A.Harada,K.Maehara,N.Horikoshi,K.Sato,Y.Sato,K.Shiraishi, N.Sugino,A.Osakabe,H.Tachiwana,W.Kagawa,H.Kimura,Y.Ohkawa, H.Kurumizaka

Key ref:

T.Urahama et al. (2016). Histone H3.5 forms an unstable nucleosome and accumulates around transcription start sites in human testis. Epigenetics Chromatin, 9, 2. PubMed id: 26779285 DOI: 10.1186/s13072-016-0051-y

Date:

03-Apr-15 Release date: 10-Feb-16

Protein chains

Q6NXT2  (H3C_HUMAN) -  Histone H3.3C from Homo sapiens

Seq: 135 a.a.

Struc: 97 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 83 a.a.

Protein chains

P04908  (H2A1B_HUMAN) -  Histone H2A type 1-B/E from Homo sapiens

Seq: 130 a.a.

Struc: 105 a.a.

Protein chains

P06899  (H2B1J_HUMAN) -  Histone H2B type 1-J from Homo sapiens

Seq: 126 a.a.

Struc: 92 a.a.

Protein chain

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 79 a.a.

DNA/RNA chains

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 146 bases

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-T-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 146 bases

DOI no: 10.1186/s13072-016-0051-y

Epigenetics Chromatin 9:2 (2016)

PubMed id: 26779285

Histone H3.5 forms an unstable nucleosome and accumulates around transcription start sites in human testis.

T.Urahama, A.Harada, K.Maehara, N.Horikoshi, K.Sato, Y.Sato, K.Shiraishi, N.Sugino, A.Osakabe, H.Tachiwana, W.Kagawa, H.Kimura, Y.Ohkawa, H.Kurumizaka.

ABSTRACT

No abstract given.