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UniProt functional annotation for P83847 | ||
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| UniProt code: P83847. |
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| Organism: | |
Geobacillus stearothermophilus (Bacillus stearothermophilus). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Geobacillus. |
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| Function: | |
Base excision repair (BER) glycosylase that initiates repair of A:oxoG to C:G by removing the inappropriately paired adenine base from the DNA backbone, generating an abasic site product (PubMed:25995449) (PubMed:14961129). 8-oxoguanine (oxoG) is a genotoxic DNA lesion resulting from oxidation of guanine; this residue is misread by replicative DNA polymerases, that insert adenine instead of cytosine opposite the oxidized damaged base. Shows a powerful dicrimination of A versus C, since it does not cleave cytosine in oxoG:C pairs (PubMed:25995449). May also be able to remove adenine from A:G mispairs, although this activity may not be physiologically relevant (PubMed:14961129). {ECO:0000269|PubMed:25995449, ECO:0000305|PubMed:14961129}. |
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| Catalytic activity: | |
Reaction=Hydrolyzes free adenine bases from 7,8-dihydro-8- oxoguanine:adenine mismatched double-stranded DNA, leaving an apurinic site.; EC=3.2.2.31; Evidence={ECO:0000269|PubMed:19841264, ECO:0000269|PubMed:25995449}; |
| Cofactor: | |
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000305|PubMed:14961129, ECO:0000305|PubMed:25995449}; Note=Binds 1 [4Fe-4S] cluster (PubMed:14961129, PubMed:25995449). The cluster has a structural role (PubMed:14961129). {ECO:0000269|PubMed:14961129, ECO:0000269|PubMed:25995449}; |
| Similarity: | |
Belongs to the Nth/MutY family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.