PDBsum entry 4yii

Ligase/cell cycle

PDB id: 4yii

Contents

Protein chains

145 a.a.

72 a.a.

Waters ×64

PDB id:

4yii

Name:

Ligase/cell cycle

Title:

Structure of an apc2-ubch10 complex reveals distinctive cullin-ring ligase mechanism for anaphase-promoting complex/cyclosome

Structure:

Ubiquitin-conjugating enzyme e2 c. Chain: u. Fragment: unp residues 27-179. Synonym: ubch10,ubiquitin carrier protein c,ubiquitin-protein ligase c. Engineered: yes. Anaphase-promoting complex subunit 2. Chain: a. Fragment: unp residues 735-822.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: ube2c, ubch10. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: anapc2, apc2, kiaa1406. Expression_system_taxid: 562

Resolution:

1.80Å R-factor: 0.199 R-free: 0.245

Authors:

N.G.Brown,S.E.Cho,B.A.Schulman

Key ref:

N.G.Brown et al. Crystal structure of e2 complex. Proc natl acad sci usa, .

Date:

02-Mar-15 Release date: 08-Apr-15

Protein chain

O00762  (UBE2C_HUMAN) -  Ubiquitin-conjugating enzyme E2 C from Homo sapiens

Seq: 179 a.a.

Struc: 145 a.a.

Protein chain

Q9UJX6  (ANC2_HUMAN) -  Anaphase-promoting complex subunit 2 from Homo sapiens

Seq: 822 a.a.

Struc: 72 a.a.

Enzyme reactions

• Enzyme class 1: Chain A: E.C.?
• Enzyme class 2: Chain U: E.C.2.3.2.23 - E2 ubiquitin-conjugating enzyme.
• Reaction: S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine
• Enzyme class 3: Chain U: E.C.2.3.2.24 - (E3-independent) E2 ubiquitin-conjugating enzyme.
• Reaction: S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E1 ubiquitin-activating enzyme]-L-cysteine + N₆- monoubiquitinyl-[acceptor protein]-L-lysine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.