PDBsum entry 4ycz

Structural protein

PDB id: 4ycz

Contents

Protein chains

713 a.a.

590 a.a.

195 a.a.

PDB id:

4ycz

Name:

Structural protein

Title:

Y-complex hub (nup85-nup120-nup145c-sec13 complex) from m. Thermophila (a.K.A. T. Heterothallica)

Structure:

Fusion protein of sec13 and nup145c. Chain: a. Engineered: yes. Nup85. Chain: b. Engineered: yes. Nup120. Chain: c. Engineered: yes

Source:

Thielavia heterothallica. Organism_taxid: 573729. Strain: atcc 42464 / bcrc 31852 / dsm 1799. Gene: mycth_2306744, mycth_2306912. Expressed in: escherichia coli. Expression_system_taxid: 511693. Gene: mycth_2059413. Gene: mycth_2296711.

Resolution:

4.10Å R-factor: 0.322 R-free: 0.358

Authors:

K.Kelley,K.E.Knockenhauer,T.U.Schwartz

Key ref:

K.Kelley et al. (2015). Atomic structure of the Y complex of the nuclear pore. Nat Struct Biol, 22, 425-431. PubMed id: 25822992 DOI: 10.1038/nsmb.2998

Date:

20-Feb-15 Release date: 01-Apr-15

Protein chain

G2QES5  (G2QES5_MYCTT) -  Protein transport protein SEC13 from Thermothelomyces thermophilus (strain ATCC 42464 / BCRC 31852 / DSM 1799)

Seq: 304 a.a.

Struc: 713 a.a.*

Protein chain

G2QEZ2  (G2QEZ2_MYCTT) -  Nuclear pore complex protein NUP96 C-terminal domain-containing protein from Thermothelomyces thermophilus (strain ATCC 42464 / BCRC 31852 / DSM 1799)

Seq: 377 a.a.

Struc: 713 a.a.*

Protein chain

G2Q7J4  (G2Q7J4_MYCTT) -  Nuclear pore complex protein Nup85 from Thermothelomyces thermophilus (strain ATCC 42464 / BCRC 31852 / DSM 1799)

Seq: 1181 a.a.

Struc: 590 a.a.

Protein chain

G2Q2S2  (G2Q2S2_MYCTT) -  Uncharacterized protein from Thermothelomyces thermophilus (strain ATCC 42464 / BCRC 31852 / DSM 1799)

Seq: 1241 a.a.

Struc: 195 a.a.

* PDB and UniProt seqs differ at 26 residue positions (black crosses)

DOI no: 10.1038/nsmb.2998

Nat Struct Biol 22:425-431 (2015)

PubMed id: 25822992

Atomic structure of the Y complex of the nuclear pore.

K.Kelley, K.E.Knockenhauer, G.Kabachinski, T.U.Schwartz.

ABSTRACT

The nuclear pore complex (NPC) is the principal gateway for transport into and out of the nucleus. Selectivity is achieved through the hydrogel-like core of the NPC. The structural integrity of the NPC depends on ~15 architectural proteins, which are organized in distinct subcomplexes to form the >40-MDa ring-like structure. Here we present the 4.1-Å crystal structure of a heterotetrameric core element ('hub') of the Y complex, the essential NPC building block, from Myceliophthora thermophila. Using the hub structure together with known Y-complex fragments, we built the entire ~0.5-MDa Y complex. Our data reveal that the conserved core of the Y complex has six rather than seven members. Evolutionarily distant Y-complex assemblies share a conserved core that is very similar in shape and dimension, thus suggesting that there are closely related architectural codes for constructing the NPC in all eukaryotes.