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PDBsum entry 4y2k
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Metal transport
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PDB id
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4y2k
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References listed in PDB file
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Key reference
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Title
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Structural insights and the surprisingly low mechanical stability of the au-S bond in the gold-Specific protein golb.
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Authors
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W.Wei,
Y.Sun,
M.Zhu,
X.Liu,
P.Sun,
F.Wang,
Q.Gui,
W.Meng,
Y.Cao,
J.Zhao.
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Ref.
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J Am Chem Soc, 2015,
137,
15358-15361.
[DOI no: ]
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PubMed id
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Abstract
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The coordination bond between gold and sulfur (Au-S) has been widely studied and
utilized in many fields. However, detailed investigations on the basic nature of
this bond are still lacking. A gold-specific binding protein, GolB, was recently
identified, providing a unique opportunity for the study of the Au-S bond at the
molecular level. We probed the mechanical strength of the gold-sulfur bond in
GolB using single-molecule force spectroscopy. We measured the rupture force of
the Au-S bond to be 165 pN, much lower than Au-S bonds measured on different
gold surfaces (∼1000 pN). We further solved the structures of apo-GolB and
Au(I)-GolB complex using X-ray crystallography. These structures showed that the
average Au-S bond length in GolB is much longer than the reported average value
of Au-S bonds. Our results highlight the dramatic influence of the unique
biological environment on the stability and strength of metal coordination bonds
in proteins.
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