PDBsum entry 4xyh

Chaperone

PDB id: 4xyh

Contents

Protein chain

375 a.a.

Waters ×144

PDB id:

4xyh

Name:

Chaperone

Title:

Wild-type full length mis16 in schizosaccharomyces japonicus

Structure:

Kinetochore protein mis16. Chain: a. Engineered: yes

Source:

Schizosaccharomyces japonicus (strain yfs275 / fy16936). Fission yeast. Organism_taxid: 402676. Strain: yfs275 / fy16936. Gene: sjag_03867. Expressed in: baculovirus expression vector pfastbac1-hm. Expression_system_taxid: 274590. Expression_system_cell_line: hi-5.

Resolution:

2.30Å R-factor: 0.177 R-free: 0.208

Authors:

S.An,H.Kim,U.-S.Cho

Key ref:

S.An et al. (2015). Mis16 Independently Recognizes Histone H4 and the CENP-ACnp1-Specific Chaperone Scm3sp. J Mol Biol, 427, 3230-3240. PubMed id: 26343758 DOI: 10.1016/j.jmb.2015.08.022

Date:

02-Feb-15 Release date: 13-Jan-16

Protein chain

B6K598  (B6K598_SCHJY) -  Kinetochore protein Mis16 from Schizosaccharomyces japonicus (strain yFS275 / FY16936)

Seq: 427 a.a.

Struc: 375 a.a.

DOI no: 10.1016/j.jmb.2015.08.022

J Mol Biol 427:3230-3240 (2015)

PubMed id: 26343758

Mis16 Independently Recognizes Histone H4 and the CENP-ACnp1-Specific Chaperone Scm3sp.

S.An, H.Kim, U.S.Cho.

ABSTRACT

CENP-A is a centromere-specific histone H3 variant that is required for kinetochore assembly and accurate chromosome segregation. For it to function properly, CENP-A must be specifically localized to centromeres. In fission yeast, Scm3sp and the Mis18 complex, composed of Mis16, Eic1, and Mis18, function as a CENP-A(Cnp1)-specific chaperone and a recruiting factor, respectively, and together ensure accurate delivery of CENP-A(Cnp1) to centromeres. Although how Scm3sp specifically recognizes CENP-A(Cnp1) has been revealed recently, the recruiting mechanism of CENP-A(Cnp1) via the Mis18 complex remains unknown. In this study, we have determined crystal structures of Schizosaccharomyces japonicus Mis16 alone and in complex with the helix 1 of histone H4 (H4α1). Crystal structures followed by mutant analysis and affinity pull-downs have revealed that Mis16 recognizes both H4α1 and Scm3sp independently within the CENP-A(Cnp1)/H4:Scm3sp complex. This observation suggests that Mis16 gains CENP-A(Cnp1) specificity by recognizing both Scm3sp and histone H4. Our studies provide insights into the molecular mechanisms underlying specific recruitment of CENP-A(Cnp1)/H4:Scm3sp into centromeres.