PDBsum entry 4xuj

DNA-binding protein/DNA

PDB id: 4xuj

Contents

Protein chains

97 a.a.

82 a.a.

106 a.a.

95 a.a.

87 a.a.

DNA/RNA

Ligands

SO4 ×3

4A6 ×3

Metals

_MG

Waters ×1

PDB id:

4xuj

Name:

DNA-binding protein/DNA

Title:

Nucleosome core particle containing adducts from treatment with a thiomorpholine-substituted [(eta-6-p-cymene)ru(3-hydroxy-2-pyridone) cl] compound

Structure:

Histone h3.2. Chain: a, e. Fragment: unp residues 2-136. Engineered: yes. Histone h4. Chain: b, f. Fragment: unp residues 2-103. Engineered: yes. Histone h2a.

Source:

Xenopus laevis. African clawed frog. Organism_taxid: 8355. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: hist1h2aj, loc494591. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Resolution:

3.18Å R-factor: 0.265 R-free: 0.299

Authors:

Z.Adhireksan,C.A.Davey

Key ref:

M.Hanif et al. Contrasting cytotoxicity and reactivity of ruii-Arene complexes of morpholine vs thiomorpholine substituted 3-Hydroxy-2-Pyridone ligands. To be published, . PubMed id: 31961568

Date:

26-Jan-15 Release date: 27-Jan-16

Protein chains

P84233  (H32_XENLA) -  Histone H3.2 from Xenopus laevis

Seq: 136 a.a.

Struc: 97 a.a.*

Protein chain

P62799  (H4_XENLA) -  Histone H4 from Xenopus laevis

Seq: 103 a.a.

Struc: 82 a.a.

Protein chains

P06897  (H2A1_XENLA) -  Histone H2A type 1 from Xenopus laevis

Seq: 130 a.a.

Struc: 106 a.a.*

Protein chains

P02281  (H2B11_XENLA) -  Histone H2B 1.1 from Xenopus laevis

Seq: 126 a.a.

Struc: 95 a.a.*

Protein chain

P62799  (H4_XENLA) -  Histone H4 from Xenopus laevis

Seq: 103 a.a.

Struc: 87 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

DNA/RNA chains

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-A-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 145 bases

A-T-C-A-A-T-A-T-C-C-A-C-C-T-G-C-A-G-A-T-A-C-T-A-C-C-A-A-A-A-G-T-G-T-A-T-T-T-G- 145 bases