PDBsum entry 4xmm

Transport protein/immune system

PDB id: 4xmm

Contents

Protein chains

274 a.a.

511 a.a.

307 a.a.

620 a.a.

896 a.a.

419 a.a.

217 a.a.

210 a.a.

PDB id:

4xmm

Name:

Transport protein/immune system

Title:

Structure of the yeast coat nucleoporin complex, space group c2

Structure:

Protein transport protein sec13. Chain: a. Engineered: yes. Nucleoporin nup145. Chain: b. Synonym: nuclear pore protein nup145. Engineered: yes. Nucleoporin seh1. Chain: c.

Source:

Saccharomyces cerevisiae s288c. Baker's yeast. Organism_taxid: 559292. Strain: s288c. Atcc: 204508. Gene: sec13, anu3, ylr208w, l8167.4. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: nup145, rat10, ygl092w.

Resolution:

7.38Å R-factor: 0.332 R-free: 0.353

Authors:

T.Stuwe,A.R.Correia,D.H.Lin,M.Paduch,V.T.Lu,A.A.Kossiakoff,A.Hoelz

Key ref:

T.Stuwe et al. (2015). Nuclear pores. Architecture of the nuclear pore complex coat. Science, 347, 1148-1152. PubMed id: 25745173 DOI: 10.1126/science.aaa4136

Date:

14-Jan-15 Release date: 25-Mar-15

Protein chain

Q04491  (SEC13_YEAST) -  Protein transport protein SEC13 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 297 a.a.

Struc: 274 a.a.

Protein chain

P49687  (NU145_YEAST) -  Nucleoporin NUP145 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1317 a.a.

Struc: 511 a.a.*

Protein chain

P53011  (SEH1_YEAST) -  Nucleoporin SEH1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 349 a.a.

Struc: 307 a.a.

Protein chain

P46673  (NUP85_YEAST) -  Nucleoporin NUP85 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 744 a.a.

Struc: 620 a.a.

Protein chain

P35729  (NU120_YEAST) -  Nucleoporin NUP120 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1037 a.a.

Struc: 896 a.a.

Protein chain

P52891  (NUP84_YEAST) -  Nucleoporin NUP84 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 726 a.a.

Struc: 419 a.a.

Protein chain

No UniProt id for this chain

Struc: 217 a.a.

Protein chain

No UniProt id for this chain

Struc: 210 a.a.

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain B: E.C.3.4.21.- - ?????

DOI no: 10.1126/science.aaa4136

Science 347:1148-1152 (2015)

PubMed id: 25745173

Nuclear pores. Architecture of the nuclear pore complex coat.

T.Stuwe, A.R.Correia, D.H.Lin, M.Paduch, V.T.Lu, A.A.Kossiakoff, A.Hoelz.

ABSTRACT

The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional nucleocytoplasmic transport. Despite half a century of structural characterization, the architecture of the NPC remains unknown. Here we present the crystal structure of a reconstituted ~400-kilodalton coat nucleoporin complex (CNC) from Saccharomyces cerevisiae at a 7.4 angstrom resolution. The crystal structure revealed a curved Y-shaped architecture and the molecular details of the coat nucleoporin interactions forming the central "triskelion" of the Y. A structural comparison of the yeast CNC with an electron microscopy reconstruction of its human counterpart suggested the evolutionary conservation of the elucidated architecture. Moreover, 32 copies of the CNC crystal structure docked readily into a cryoelectron tomographic reconstruction of the fully assembled human NPC, thereby accounting for ~16 megadalton of its mass.