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PDBsum entry 4xmm
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Transport protein/immune system
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PDB id
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4xmm
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Contents |
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274 a.a.
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511 a.a.
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307 a.a.
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620 a.a.
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896 a.a.
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419 a.a.
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217 a.a.
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210 a.a.
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References listed in PDB file
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Key reference
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Title
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Nuclear pores. Architecture of the nuclear pore complex coat.
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Authors
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T.Stuwe,
A.R.Correia,
D.H.Lin,
M.Paduch,
V.T.Lu,
A.A.Kossiakoff,
A.Hoelz.
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Ref.
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Science, 2015,
347,
1148-1152.
[DOI no: ]
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PubMed id
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Abstract
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The nuclear pore complex (NPC) constitutes the sole gateway for bidirectional
nucleocytoplasmic transport. Despite half a century of structural
characterization, the architecture of the NPC remains unknown. Here we present
the crystal structure of a reconstituted ~400-kilodalton coat nucleoporin
complex (CNC) from Saccharomyces cerevisiae at a 7.4 angstrom resolution. The
crystal structure revealed a curved Y-shaped architecture and the molecular
details of the coat nucleoporin interactions forming the central
"triskelion" of the Y. A structural comparison of the yeast CNC with
an electron microscopy reconstruction of its human counterpart suggested the
evolutionary conservation of the elucidated architecture. Moreover, 32 copies of
the CNC crystal structure docked readily into a cryoelectron tomographic
reconstruction of the fully assembled human NPC, thereby accounting for ~16
megadalton of its mass.
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