PDBsum entry 4xls

Transcription

PDB id: 4xls

Contents

Protein chains

227 a.a.

1112 a.a.

1490 a.a.

93 a.a.

345 a.a.

1367 a.a.

162 a.a.

DNA/RNA

Metals

_MG ×2

_ZN ×4

PDB id:

4xls

Name:

Transcription

Title:

Crystal structure of t. Aquaticus transcription initiation complex with card containing upstream fork promoter.

Structure:

DNA-directed RNA polymerase subunit alpha. Chain: a, b, g, h. Synonym: rnap subunit alpha,RNA polymerase subunit alpha, transcriptase subunit alpha. DNA-directed RNA polymerase subunit beta. Chain: c, i. Synonym: rnap subunit beta,RNA polymerase subunit beta,transcriptase subunit beta. DNA-directed RNA polymerase subunit beta'.

Source:

Thermus aquaticus. Organism_taxid: 271. Other_details: cell. Gene: siga. Expressed in: escherichia coli. Expression_system_taxid: 469008. Thermus thermophilus jl-18. Organism_taxid: 798128. Gene: ttjl18_0238.

Resolution:

4.01Å R-factor: 0.234 R-free: 0.272

Authors:

B.Bae,S.A.Darst

Key ref:

B.Bae et al. (2015). Card uses a minor groove wedge mechanism to stabilize RNA polymerase open promoter complex.. Elife, 4, . PubMed id: 26349034

Date:

13-Jan-15 Release date: 23-Sep-15

Protein chains

Q9KWU8  (RPOA_THEAQ) -  DNA-directed RNA polymerase subunit alpha from Thermus aquaticus

Seq: 314 a.a.

Struc: 227 a.a.

Protein chains

Q9KWU7  (RPOB_THEAQ) -  DNA-directed RNA polymerase subunit beta from Thermus aquaticus

Seq: 1119 a.a.

Struc: 1112 a.a.

Protein chain

Q9KWU6  (RPOC_THEAQ) -  DNA-directed RNA polymerase subunit beta' from Thermus aquaticus

Seq: 1524 a.a.

Struc: 1490 a.a.

Protein chains

Q9EVV4  (RPOZ_THEAQ) -  DNA-directed RNA polymerase subunit omega from Thermus aquaticus

Seq: 99 a.a.

Struc: 93 a.a.

Protein chains

Q9EZJ8  (SIGA_THEAQ) -  RNA polymerase sigma factor SigA from Thermus aquaticus

Seq: 438 a.a.

Struc: 345 a.a.

Protein chain

Q9KWU6  (RPOC_THEAQ) -  DNA-directed RNA polymerase subunit beta' from Thermus aquaticus

Seq: 1524 a.a.

Struc: 1367 a.a.

Protein chains

H9ZP94  (H9ZP94_THETH) -  CarD-like transcriptional regulator from Thermus thermophilus JL-18

Seq: 164 a.a.

Struc: 162 a.a.

DNA/RNA chains

C-T-T-G-A-C-A-A-A-A-G-T-G-T-T-A-A-A-T-T-G-T-G-C-T-A-T-A-C-T 30 bases

G-C-A-C-A-A-T-T-T-A-A-C-A-C-T-T-T-T-G-T-C-A-A-G 24 bases

C-T-T-G-A-C-A-A-A-A-G-T-G-T-T-A-A-A-T-T-G-T-G-C-T-A-T-A-C-T 30 bases

G-C-A-C-A-A-T-T-T-A-A-C-A-C-T-T-T-T-G-T-C-A-A-G 24 bases

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, G, H, I, J, K: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site