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PDBsum entry 4xhv
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Signaling protein
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PDB id
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4xhv
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PDB id:
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| Name: |
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Signaling protein
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Title:
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Crystal structure of drosophila spinophilin-pdz and a c-terminal peptide of neurexin
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Structure:
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Lp20995p. Chain: a. Fragment: unp residues 1258-1347. Engineered: yes. Neurexin 1. Chain: b. Engineered: yes
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Source:
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Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Gene: spn-ra. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 7227
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Resolution:
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1.23Å
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R-factor:
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0.139
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R-free:
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0.162
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Authors:
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J.H.Driller,K.G.H.Muhammad,S.Reddy,U.Rey,M.A.Boehme,C.Hollmann, N.Ramesh,H.Depner,J.Luetzkendorf,T.Matkovic,D.Bergeron,C.Quentin, J.Schmoranzer,F.Goettfert,M.Holt,M.C.Wahl,S.W.Hell,A.Walter, S.J.Sigrist,B.Loll
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Key ref:
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K.Muhammad
et al.
(2015).
Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function.
Nat Commun,
6,
8362.
PubMed id:
DOI:
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Date:
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06-Jan-15
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Release date:
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01-Jul-15
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PROCHECK
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Headers
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References
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M9NFI9
(M9NFI9_DROME) -
Spinophilin, isoform J from Drosophila melanogaster
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Seq:
Struc:
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2148 a.a.
94 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 4 residue positions (black
crosses)
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DOI no:
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Nat Commun
6:8362
(2015)
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PubMed id:
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Presynaptic spinophilin tunes neurexin signalling to control active zone architecture and function.
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K.Muhammad,
S.Reddy-Alla,
J.H.Driller,
D.Schreiner,
U.Rey,
M.A.Böhme,
C.Hollmann,
N.Ramesh,
H.Depner,
J.Lützkendorf,
T.Matkovic,
T.Götz,
D.D.Bergeron,
J.Schmoranzer,
F.Goettfert,
M.Holt,
M.C.Wahl,
S.W.Hell,
P.Scheiffele,
A.M.Walter,
B.Loll,
S.J.Sigrist.
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ABSTRACT
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Assembly and maturation of synapses at the Drosophila neuromuscular junction
(NMJ) depend on trans-synaptic neurexin/neuroligin signalling, which is promoted
by the scaffolding protein Syd-1 binding to neurexin. Here we report that the
scaffold protein spinophilin binds to the C-terminal portion of neurexin and is
needed to limit neurexin/neuroligin signalling by acting antagonistic to Syd-1.
Loss of presynaptic spinophilin results in the formation of excess, but
atypically small active zones. Neuroligin-1/neurexin-1/Syd-1 levels are
increased at spinophilin mutant NMJs, and removal of single copies of the
neurexin-1, Syd-1 or neuroligin-1 genes suppresses the spinophilin-active zone
phenotype. Evoked transmission is strongly reduced at spinophilin terminals,
owing to a severely reduced release probability at individual active zones. We
conclude that presynaptic spinophilin fine-tunes neurexin/neuroligin signalling
to control active zone number and functionality, thereby optimizing them for
action potential-induced exocytosis.
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