PDBsum entry 4xf2

Structural protein

PDB id: 4xf2

Contents

Protein chains

392 a.a.

228 a.a.

354 a.a.

284 a.a.

174 a.a.

167 a.a.

139 a.a.

PDB id:

4xf2

Name:

Structural protein

Title:

Tetragonal structure of arp2/3 complex

Structure:

Actin-related protein 3. Chain: a, t. Synonym: actin-2,actin-like protein 3. Actin-related protein 2. Chain: b, u. Synonym: actin-like protein 2. Actin-related protein 2/3 complex subunit 1b. Chain: c, v. Synonym: arp2/3 complex 41 kda subunit,p41-arc.

Source:

Bos taurus. Bovine. Organism_taxid: 9913. Organ: thymus. Organ: thymus

Resolution:

5.00Å R-factor: 0.276 R-free: 0.303

Authors:

C.T.Jurgenson,T.P.Pollard

Key ref:

C.T.Jurgenson and T.D.Pollard (2015). Crystals of the Arp2/3 complex in two new space groups with structural information about actin-related protein 2 and potential WASP binding sites. Acta Crystallogr F Struct Biol Commun, 71, 1161-1168. PubMed id: 26323303 DOI: 10.1107/S2053230X15013515

Date:

25-Dec-14 Release date: 18-Nov-15

Protein chains

P61157  (ARP3_BOVIN) -  Actin-related protein 3 from Bos taurus

Seq: 418 a.a.

Struc: 392 a.a.

Protein chains

A7MB62  (ARP2_BOVIN) -  Actin-related protein 2 from Bos taurus

Seq: 394 a.a.

Struc: 228 a.a.*

Protein chains

Q58CQ2  (ARC1B_BOVIN) -  Actin-related protein 2/3 complex subunit 1B from Bos taurus

Seq: 372 a.a.

Struc: 354 a.a.*

Protein chains

Q3MHR7  (ARPC2_BOVIN) -  Actin-related protein 2/3 complex subunit 2 from Bos taurus

Seq: 300 a.a.

Struc: 284 a.a.

Protein chains

Q3T035  (ARPC3_BOVIN) -  Actin-related protein 2/3 complex subunit 3 from Bos taurus

Seq: 178 a.a.

Struc: 174 a.a.

Protein chains

Q148J6  (ARPC4_BOVIN) -  Actin-related protein 2/3 complex subunit 4 from Bos taurus

Seq: 168 a.a.

Struc: 167 a.a.

Protein chains

Q3SYX9  (ARPC5_BOVIN) -  Actin-related protein 2/3 complex subunit 5 from Bos taurus

Seq: 151 a.a.

Struc: 139 a.a.*

* PDB and UniProt seqs differ at 18 residue positions (black crosses)

DOI no: 10.1107/S2053230X15013515

Acta Crystallogr F Struct Biol Commun 71:1161-1168 (2015)

PubMed id: 26323303

Crystals of the Arp2/3 complex in two new space groups with structural information about actin-related protein 2 and potential WASP binding sites.

C.T.Jurgenson, T.D.Pollard.

ABSTRACT

Co-crystals of the bovine Arp2/3 complex with the CA motif from N-WASP in two new space groups were analyzed by X-ray diffraction. The crystals in the orthorhombic space group P212121 contained one complex per asymmetric unit, with unit-cell parameters a = 105.48, b = 156.71, c = 177.84 Å, and diffracted to 3.9 Å resolution. The crystals in the tetragonal space group P41 contained two complexes per asymmetric unit, with unit-cell parameters a = b = 149.93, c = 265.91 Å, and diffracted to 5.0 Å resolution. The electron-density maps of both new crystal forms had densities for small segments of subdomains 1 and 2 of Arp2. Both maps had density at the binding site on Arp3 for the C-terminal EWE tripeptide from N-WASP and a binding site proposed for the C motif of N-WASP in the barbed-end groove of Arp2. The map from the tetragonal crystal form had density near the barbed end of Arp3 that may correspond to the C helix of N-WASP. The noise levels and the low resolution of the maps made the assignment of specific molecular structures for any of these CA peptides impossible.