PDBsum entry 4xei

Structural protein

PDB id: 4xei

Contents

Protein chains

400 a.a.

218 a.a.

344 a.a.

282 a.a.

174 a.a.

168 a.a.

135 a.a.

Waters ×48

References listed in PDB file

Key reference

• Title: Crystals of the arp2/3 complex in two new space groups with structural information about actin-Related protein 2 and potential wasp binding sites.
• Authors: C.T.Jurgenson, T.D.Pollard.
• Ref.: Acta Crystallogr F Struct Biol Commun, 2015, 71, 1161-1168. [DOI no: 10.1107/S2053230X15013515]
• PubMed id: 26323303

Note: In the PDB file this reference is annotated as "TO BE PUBLISHED". The citation details given above have been manually determined.

Abstract

Co-crystals of the bovine Arp2/3 complex with the CA motif from N-WASP in two new space groups were analyzed by X-ray diffraction. The crystals in the orthorhombic space group P212121 contained one complex per asymmetric unit, with unit-cell parameters a = 105.48, b = 156.71, c = 177.84 Å, and diffracted to 3.9 Å resolution. The crystals in the tetragonal space group P41 contained two complexes per asymmetric unit, with unit-cell parameters a = b = 149.93, c = 265.91 Å, and diffracted to 5.0 Å resolution. The electron-density maps of both new crystal forms had densities for small segments of subdomains 1 and 2 of Arp2. Both maps had density at the binding site on Arp3 for the C-terminal EWE tripeptide from N-WASP and a binding site proposed for the C motif of N-WASP in the barbed-end groove of Arp2. The map from the tetragonal crystal form had density near the barbed end of Arp3 that may correspond to the C helix of N-WASP. The noise levels and the low resolution of the maps made the assignment of specific molecular structures for any of these CA peptides impossible.