PDBsum entry 4x9j

Transcription regulator/DNA

PDB id: 4x9j

Contents

Protein chain

86 a.a.

DNA/RNA

Metals

_ZN ×3

Waters ×208

PDB id:

4x9j

Name:

Transcription regulator/DNA

Title:

Egr-1 with doubly methylated DNA

Structure:

Early growth response protein 1. Chain: a. Fragment: unp residues 335-423. Synonym: egr-1,at225,nerve growth factor-induced protein a,ngfi-a, transcription factor etr103,transcription factor zif268,zinc finger protein 225,zinc finger protein krox-24. Engineered: yes. DNA (5'-d( Ap Gp (5Cm)p Gp Tp Gp Gp Gp (5Cm)p Gp T)-3'). Chain: b.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: egr1, krox24, znf225. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Resolution:

1.41Å R-factor: 0.174 R-free: 0.200

Authors:

M.A.White,L.Zandarashvili,J.Iwahara

Key ref:

L.Zandarashvili et al. (2015). Structural impact of complete CpG methylation within target DNA on specific complex formation of the inducible transcription factor Egr-1. Febs Lett, 589, 1748-1753. PubMed id: 25999311 DOI: 10.1016/j.febslet.2015.05.022

Date:

11-Dec-14 Release date: 13-May-15

Protein chain

P18146  (EGR1_HUMAN) -  Early growth response protein 1 from Homo sapiens

Seq: 543 a.a.

Struc: 86 a.a.

DNA/RNA chains

A-G-5CM-G-T-G-G-G-5CM-G-T 11 bases

T-A-5CM-G-C-C-C-A-5CM-G-C 11 bases

DOI no: 10.1016/j.febslet.2015.05.022

Febs Lett 589:1748-1753 (2015)

PubMed id: 25999311

Structural impact of complete CpG methylation within target DNA on specific complex formation of the inducible transcription factor Egr-1.

L.Zandarashvili, M.A.White, A.Esadze, J.Iwahara.

ABSTRACT

No abstract given.